Phenomenex, Inc.

Using the Clarity OTX extraction protocol vendor recommendations, we have demonstrated its use to develop a method for the extraction of therapeutic oligos from plasma.

This technical note discusses 2' cleavage and RNA sample preparation prior to trityl-on Clarity QSP cartridge or high-throughput purification.

As synthetic oligonucleotide therapeutics traverse the drug-development path, there is an increasing need for TK and PK/PD studies in drug discovery and development.

Common frequently asked question for oligonucleotides. This includes questions about characterization to purification and from sample preparation to LC separation.

This technical note discusses a simple, yet quick and effective solution for isolating and analyzing therapeutic oligonucleotides using Clarity OTX, an extraction kit.

Advance your oligonucleotide work, from sample preparation to analysis and purification

To ensure recovery and reproducibility for oligo extraction from tissue, it is imperative that an effective sample pretreatment strategy is used.

Clarity QSP provides an alternative trityl-on approach for separating truncated sequences and synthetic contaminants from the protected full-length sequence.

In this technical note we explore column miniaturization effects attained by reducing column ID from 300 μm to 75 μm and its effects on sensitivity.

Impact of sub-2 μm fully porous particles and core-shell particles in nano LC–MS evaluated by separation performance and protein and peptide identifications in proteomics.

How to achieve deep proteome coverage on HeLa cell lysate using a high pH reversed phase fractionation strategy in combination with nano flow LC–MS

In this application note we explore the effects of trap loadability using a bioZen 3 μm Polar C18 nano column in conjunction with the bioZen RP1 (C18) nano trap column.

In this technical note we show peptides and proteins identified using the bioZen nano XB-C18 column in either direct inject or trap and elute mode.

The effect of column selectivity and its importance in the quality of data achieved in protein and peptide identifications in bottom-up proteomics applications is demonstrated.

In this application, we show reproducible results across multiple columns as well as the robust ability of the bioZen 2.6 μm XB-C18 column after 100 injections.

A miniaturized LC–MS and SPE method for the reliable and accurate detection of viral pesticides at 1 fmol/uL using SARS-CoV-2 nucleocapsid protein as a model system.

***Live: Wednesday, March 3, 2021 at 11am EST | 8am PST | 4pm GMT | 5pm CET *** In this presentation we will demonstrate the gains in proteome characterization that can be achieved by using modern HPLC materials, both core–shell and full porous based. We will also show improvements that can be realized via selectivity optimization of not only the HPLC column chemistry but also trap chemistries when using a trap-and-elute workflow. .***On Demand until Mar. 3, 2022***

A SLE application for acids (THC-COOH), neutrals (barbiturates), and bases (Norbuprenorphine and Buprenorphine) from a urine matrix containing β-glucuronidase followed by two LC-MS/MS methods.

Estrone, beta-estradiol, and estriol were derivatized from plasma using Novum PRO 96-Well Plates SLE for less background noise and cleaner samples post sample preparation.

This application focuses on developing a rapid SPE and LC-MS/MS method for the quantitative analysis of uracil and its two homologues from human serum

Testosterone was isolated from plasma using Novum PRO 96-Well Plates SLE sample preparation.

Analysis of GHB demands for an analytical procedure that is quick, efficient, and one that preserves the authenticity of the sample. A method for the quantitation of GHB in human blood is presented using SPE with LC-MS/MS analysis.

In this application, an SPE method is implemented for a panel of pain relievers to produce acceptable results to offer an alternative solution for high recoveries and to reduce matrix effects.

A sensitive method for analyzing nicotine and metabolites using SLE to increase recoveries, while still displaying accuracy, precision and linearity.

In this panel of veterninary drugs from a milk sample, SPE displays an improved solution over traditional protein precipitation methods and other types of SPE, due to clean up efficiency, all while maintaining a rapid and fast analysis time.

Peptides are extracted from plasma comparing Strata-X PRO SPE to two types of ion exchange polymeric sorbent. Strata-X PRO provides high recoveries with a fast and effective clean-up method.