Phenomenex, Inc.

A novel LC approach to manipulate the chromatography of closely related PFAS analytes to separate co-eluties and avoid matrix interferences.

Wednesday, August 18, 2021 at 11am EDT | 8am PDT | 4pm BST | 5pm CEST Join us to learn how to implement easy sample preparation strategies for LC–MS/MS bioanalysis of pain management drugs and similar types of assays in difficult matrices.

Multivitamin standards were prepared and filtered using 2-step Verex Filter Vials.

Hydrocortisone was extracted from a topical cream and filtered using Verex Filter Vials. Samples were run by LC–UV to see if using filter vials created any loss of sample.

IgG1 (Trastuzumab) and an IgG4 (Nivolumab) are digested with IdeZ protease, yielding Fab2’ and scFc fragments that were filtered using Verex Filter Vials to determine if there was a loss of sample.

In this application, Methylmalonic Acid (MMA) samples were filtered and tested to determine that no extractables were introduced by using the Verex Filter Vials.

Polychlorinated Biphenyls (PCBs) were prepared and filtered using Verex Filter Vials and traditional syringe filters.

In this application, atorvastatin standards were prepared and filtered using Verex Filter Vials. Samples were analyzed by LC–UV.

Chili powder and cumin powder were analyzed for pesticides and clean-up using Verex Filter Vials to remove undissolved sample particulates.

This application guide provides a comprehensive collection of simple sample preparation applications using filtration.

Wednesday, July 7, 2021 at 11am EDT | 8am PDT | 4pm BST | 5pm CEST

Monday, June 28, 2021 at 11am EDT | 8am PDT | 4pm BST | 5pm CEST The goal of any chromatographic separation is the resolution of different analyte peaks from those with similar elution profiles. Join us to learn the three critical factors that govern the resolution of a critical pair.

Advance your oligonucleotide work, from sample preparation to analysis and purification.

This technical note discusses 2' cleavage and RNA sample preparation prior to trityl-on Clarity QSP cartridge or high-throughput purification.

Using the Clarity OTX extraction protocol vendor recommendations, we have demonstrated its use to develop a method for the extraction of therapeutic oligos from plasma.

Common frequently asked question for oligonucleotides. This includes questions about characterization to purification and from sample preparation to LC separation.

Clarity QSP provides an alternative trityl-on approach for separating truncated sequences and synthetic contaminants from the protected full-length sequence. By incorporating a polymeric sorbent with a unique buffer formula, aborted fragments and synthetic contaminants are removed during sample loading offering a departure from the traditional RPC mechanism. Multiple buffers and gradient elutions are eliminated, thereby making Clarity QSP a 3-step product for trityl-on oligonucleotide purification.

As synthetic oligonucleotide therapeutics move through the drug-development path, the need for TK and PK/PD studies in drug discovery and development has increased.

Simple, quick, effective solution for isolating and analyzing therapeutic oligonucleotides using Clarity OTX, an extraction kit based on mixed-mode ion-exchange SPE.

To ensure recovery and reproducibility for oligo extraction from tissue, it is imperative that an effective sample pretreatment strategy is used.

Trityl-on methodologies for the analysis of crude synthetic oligonucleotides has many benefits however depurination during trityl-on methodologies can lead to nucleic acid damage. This technical note explores a new trityl-on procedure that minimizes the risk of depurination.

Extraction of Oligo therapeutics from biological samples for LC/MS bioanalysis.

Monday, June 21, 2021 at 11am EDT | 8am PDT | 4pm BST | 5pm CEST

Estrone, beta-estradiol, and estriol were derivatized from plasma using Novum PRO 96-Well Plates SLE to provide less background noise.

In this application, serum was cleaned up and compared with both a single protein precipitation protocol and also by the addition of a phospholipid removal product.

Miniaturized LC–MS and SPE method for the reliable and accurate detection of viral pesticides at 1 fmol/uL using SARS-CoV-2 nucleocapsid protein as a model system.

A quantitative LC–MS/MS method using a room-temperature hydrolysis with an advanced SPE product for the analysis of designer and pharmaceutical benzodiazepines in urine

An in-well hydrolysis solid-phase extraction (SPE) product is used for an easy clean-up solution for the urine sample matrix to save time and transfer steps.

Testosterone was isolated from plasma using Novum PRO 96-well plates SLE sample preparation.

An SLE media provides a fast sample prep method. Adding advanced manufacturing processes leads to high recoveries and low variation between samples.