Phenomenex, Inc.

In this application note we show the separation of charge variants of Trastuzumab under native conditions using a pH gradient formed with volatile buffers.

In this application note we look at a method for determining the level of aggregation in a commercially available sample of intact NIST mAb using a bioZen SEC-2 column together with the SCIEX X500B mass spec.

In this application we show the separation of several charge variants of the NIST mAb under native ion exchange and mass spectrometry (high resolution MS) conditions using a pH gradient formed from volatile buffers.

In this application note we show high resolution separation for a suite of protein standards under native mass spec conditions typical for SEC.

In this application note we show high resolution separation for a suite of protein standards under native mass spec conditions typical for SEC.

This application note serves to highlight how native MS can be used to get intact mass for non-covalently linked large molecules, including cysteine linked ADCs

Combining the efficient immunocapture and sample extraction of bioZen MagBeads, trastuzumab is isolated from rat plasma to yields greater selectivity relative to traditional ligand-binding assays.

Combining the efficient immunocapture and sample extraction of bioZen MagBeads, trastuzumab is isolated from rat plasma to yields greater selectivity relative to traditional ligand-binding assays.

In this application, Streptavidin coated bioZen MagBeads are utilized for the fast and accurate immunocapture of insulin aspart novolog. By combining immunocapture sample preparation with LC MS/MS, this method demonstrates a functionalized assay that yields greater selectivity and sensitivity.

In this application, Streptavidin coated bioZen MagBeads are utilized for the fast and accurate immunocapture of insulin aspart novolog. By combining immunocapture sample preparation with LC MS/MS, this method demonstrates a functionalized assay that yields greater selectivity and sensitivity.

In this application we show the separation of several charge variants of the NIST mAb under native ion exchange and mass spectrometry (high resolution MS) conditions using a pH gradient formed from volatile buffers.

While biological samples often are considered difficult to prepare and analyze, new advances can help considerably to ease common pain points in sample preparation to increase productivity and produce reliable results. In this webcast, Matt Brusius will educate participants on the best techniques for biological samples all while providing reliable, highly sensitive results for each technique. Live: Thursday, Aug. 29, 2019 at 11am EDT | 8am PDT | 4pm BST | 5pm CEST On demand available after airing until Aug. 29, 2020 Register free

A straightforward separation of 10 closely related forms of vitamin E with a Kinetex 2.6 µm F5 HPLC column and under reversed-phase conditions. The 10 closely related structures were chromatographically separated by the multiple selectivity interaction mechanisms of the Kinetex F5 phase and Water/Methanol mobile phase.

This method presents the reversed-phase retention and detection of 7 polar Neonicotinoids and 4 deuterated-internal standards by LC-MS/MS and with a Kinetex 2.6 Biphenyl HPLC column. The Kinetex Biphenyl phase demonstrated significant polar compound retention and selectivity.

Described in this application note are the details for an investigation of basic compound peak shape across four different alkyl C18 stationary phases that are all based upon the same core-shell HPLC/UHPLC particle morphology.

In this application, is the investigation into the relative selectivity of four different C18 stationary phases when applied to the reversed phase retention of six extremely polar artificial sweeteners.

For this analysis, we developed a fast, robust, and efficient separation on a Kinetex 2.6µm Polar C18 phase. This is a highly robust, core-shell stationary phase capable of providing increased efficiency over fully porous products. The Kinetex Polar C18 functionality provides unique polar selectivity that was critical in effectively retaining and separating these phenolic antioxidants.

For Developed is a rapid method analyzing 16 major cannabinoids with great resolution and low backpressures.

A straightforward separation of 10 closely related forms of vitamin E with a Kinetex 2.6 µm F5 HPLC column and under reversed-phase conditions. The 10 closely related structures were chromatographically separated by the multiple selectivity interaction mechanisms of the Kinetex F5 phase and Water/Methanol mobile phase.

For this application study, the alkaloid compound berberine was selected to help demonstrate the Kinetex PS C18 HPLC/UHPLC column’s unique multi-modal selectivity and improved chromatographic performance when applied to the analysis of polar basic compounds.

For this application study, the alkaloid compound berberine was selected to help demonstrate the Kinetex PS C18 HPLC/UHPLC column’s unique multi-modal selectivity and improved chromatographic performance when applied to the analysis of polar basic compounds.

A HPLC/UHPLC poster that ranks different stationary phases by 5 primary mechanism of reversed-phase selectivity.

This application is an alternative HPLC method for the analysis of 13 carbonyls without the use of any derivatization agent. The method uses the combined polar and non-polar selectivity of a Kinetex Biphenyl stationary phase to achieve improved chromatographic results.

For this analysis, we developed a fast, robust, and efficient separation on a Kinetex 2.6µm Polar C18 phase. This is a highly robust, core-shell stationary phase capable of providing increased efficiency over fully porous products. The Kinetex Polar C18 functionality provides unique polar selectivity that was critical in effectively retaining and separating these phenolic antioxidants.

This application is an alternative HPLC method for the analysis of 13 carbonyls without the use of any derivatization agent. The method uses the combined polar and non-polar selectivity of a Kinetex Biphenyl stationary phase to achieve improved chromatographic results.