Phenomenex, Inc.

In this application note, we explore arginine as a mobile phase cosolvent that improves high molecular weight aggregate recovery for size exclusion chromatography.

This guide provides an overview of the Biozen size exclusion chromatography columns for the analysis of monoclonal antibodies, biosimilars, and other biomolecules.

The versatile selectivity and high temperature limit of Zebron ZB-624plus provides a one column GC-FID solution to cannabis residual solvent and terpenes testing.

An expanded list of 15 mycotoxins for LC–MS/MS analysis is shown with a simple solvent and modified QuEChERS salt extraction. The workflow is simple and accessible.

The California list covers a wide range of pesticides with diverse chemical properties. This LC–MS/MS method features increased resolving power for the multiresidue pesticides of interest.

Flash chromatography is widely used for purifications in pharmaceutical product synthesis and is now finding similar application in CBD -THC remediation.

A practical GC-FID method for 20 terpenes from cannabis using a Zebron ZB-624PLUS GC column with an upper temperature limit of 300/320 °C.

CBG-A dominant cultivars show lower total THC concentrations compared to CBD-A dominant cultivars and are sought for their likelihood to pass total THC compliance testing.

A practical chromatographic separation of 18 cannabinoids with flexibility across HPLC and UHPLC platforms in a simple, isocratic workflow.

The use of sub-2 µm particles for SEC have expanded analytical method for the characterization of biologics. This is especially noteworthy with monoclonal antibodies (mAbs), wherein methods developed on ultra high-performance SEC (UHP-SEC) columns can separate not only aggregates, but also fragment. This application note explores the effect of column internal diameter for robust size exclusion chromatography methods.

In this application note, NIST mAb RM 8671 was analyzed using 6 different batches of 150 mm length, 4.6 mm inner diameter columns packed with 1.8 µm 200 Å SEC media.

Flow rate is a common method parameter for most adsorptive LC methods. However, SEC is unique in that lower flow rates often yield improvements in separation, and resolution can improve considerably simply by running at a lower linear velocity. This application note demonstrates this approach for the aggregate analysis of NIST mAb, a common standard protein.

Assessing phosphate concentration may be necessary to ensure that no differences are observed with modulating concentration. This ensures proper sample recovery and demonstrates robustness of the method. In this application note, we review SEC chromatographic performance for trastuzumab and NIST mAb modulating concentration of potassium phosphate.

In this application note we explore the use of sub-2-µm particles for SEC methods as a practical way to increase throughput for applications that demand it.

Size exclusion chromatography, or SEC, is a technique for separating proteins and other complex biomolecules in their native forms by size using aqueous eluents. For molecules of specific molecular weight, SEC can use used to detect and quantitate aggregates, fragments, monomers and dimer. This guide provides a comprehensive application overview for Biozen dSEC Size Exclusion Columns.

In this application note, we demonstrate the effect of organic solvent on SEC analysis of an ADC “mimic.” This sample is a monoclonal antibody that has been conjugated to a dansyl group and functions as a surrogate for the typically cytotoxic agent commonly used for most ADC modalities.

In this application note, we demonstrate the use of SEC-HRMS for the characterization of NIST mAb RM 8671, a commonly used standard monoclonal antibody surrogate for method development.

In this application note, we demonstrate the use of SEC-HRMS for the analysis of an IdeZ-digested mAb.

Size exclusion chromatography, or SEC, is a technique for separating proteins and other complex biomolecules in their native forms by size using aqueous eluents. For molecules of specific molecular weight, SEC can use used to detect and quantitate aggregates, fragments, monomers and dimer. This guide provides an overview of the Biozen SEC Size Exclusion Chromatography Columns for the analysis of monoclonal antibodies, biosimilars and other biomolecules

Thursday, August 26, 2021 at 11am EDT | 8am PDT | 4pm BST | 5pm CEST Developing a robust, transferable size-exclusion chromatography (SEC) method for routine testing of mabs and other biotherapeutics can be challenging. Join us for this presentation to learn about SEC method development, with an emphasis on method transfer and sustainability.

Application guide to PFAS analysis in environmental and food samples, from sample preparation to LC–MS/MS analysis, featuring both validated and exploratory methods.

In a commercial laboratory setting, Strata-X-AW and Gemini 3 µm C18 show excellent precision and recovery in EPA Method 533 for the analysis of PFAS in drinking water.

Strata PFAS (WAX and GCB combined in a single SPE tube) compliant with DOD QSM 5.1, but more accurate, precise, and economical than dual tube or dispersed GCB solutions.

A QuEChERs + SPE sample preparation technique coupled with LC–MS/MS is shown to provide sub-ppb analysis of PFAS in dairy and fish samples.

Validation of a water and wastewater method for the California PFAS panel using SPE (WAX/GCB) preparation and LC–MS/MS analysis with a variable pH LC mobile phase

Phenomenex announces a new graphitized carbob black (GCB) SPE tube for DOD QSM 5.3 compliant PFAS analysis.

A municipal wastewater laboratory demonstrates the use of QuEChERs sample preparation techniques to analyze PFAS in sediments at sub ng/g levels.

A municipal wastewater laboratory demonstrates the use of QuEChERs sample preparation techniques to analyze PFAS in sediments at sub ng/g levels.

A wide variety of LC column chemistries were evaluated to demonstrate new chromatographic approaches to complex PFAS analyte lists.

Strata-SDB-L and Luna Omega 1.6 µm PS C18 demonstrate excellent precision and recovery in EPA Method 537.1 for the analysis of PFAS in drinking water.