Advancing Ion Chromatography with High Pressure
February 10th 2016High pressures and columns with small particle sizes have been used in high-performance liquid chromatography (HPLC) for a long time, but not in ion chromatography (IC). But now, high pressures are coming to IC. Read this new e-book to learn about: • The advantages of high-pressure and small-particle-size columns in IC • How those advantages apply to real-world examples in the analysis of food, beverages, and environmental samples • The reasons why the move to high-pressures in IC has taken so long • The drivers for the trend toward higher-capacity IC columns . . . and more! Download the e-book today!
Pushing the Boundaries of Proteomics With the New Orbitrap Tribrid Mass Spectrometer
August 6th 2015Dr. Graeme McAlister of Thermo Fisher Scientific will discuss the advances in Orbitrap Tribrid Mass Spectrometry technology with the launch of the new Thermo ScientificTM Orbitrap FusionTM LumosTM TribridTM Mass Spectrometer. Graeme will also touch on how these advances have impacted and will continue to impact proteomics with respect to quantitation, accuracy, throughput, etc.
HRMS in Clinical Research from Targeted Quantitation to Metabolomics
August 3rd 2015In this webinar, we will discuss the use of new, affordable HRMS instruments in quantitative clinical research analyses, demonstrating the sensitivity and selectivity of these instruments for this work. Examples of clinical research quantification work in HR full scan or MS-MS mode will be presented and compared with results from triple-quadrupole-MS. We will also discuss an example of Qual/Quan analysis in a study of the fate of an anti-cancer agent in humans, where over 40 metabolites were identified and quantified. We will also show metabolomics data underscoring the versatility of HRMS instruments.
Analytical Methods to Characterize and Quantify PEG and PEGylated Biopharmaceuticals
June 1st 2012Biotherapeutic peptides and proteins are often PEGylated (covalently bonded to polyethylene glycol polymers) to improve bioavailability, reduce immunogenicity, and extend circulating half-life (1). Achieving the desired properties for each application depends on optimizing the number and site of polymers attached, chain length, and the degree of chain branching.
Glycan Analysis using HPLC with Charged Aerosol Detection and MS Detectors
June 1st 2012Simple, rapid and accurate methods have been developed for the analysis of simple carbohydrates including mono- or disaccharides, or as fairly small glycans (6–11 residues) liberated from glycoproteins using charged aerosol detection combined with mass spectrometry techniques.
Faster, More Sensitive Determination of Carbamates in Drinking Water
February 1st 2012U.S. EPA Method 531.2 describes the determination of widely used carbamate pesticides in raw surface water using HPLC with fluorescence detection following postcolumn derivatization, which enhances method sensitivity and selectivity as compared to UV absorbance detection.
LC–MS-MS Method for the Determination of Enalapril and Enalaprilat fro Human Plasma Using SOLA
February 1st 2012A liquid chromatography tandem mass spectrometry method for enalapril and enalaprilat from human plasma has been developed using Thermo Scientific SOLA cartridges. Sample preparation is fast, efficient, and reproducible giving excellent recovery levels for each compound.
New HPLC/UHPLC Assay Methods for Impurities in Tetracycline
February 1st 2012Tetracycline (TC), a common antibiotic used to treat urinary tract infections, acne, gonorrhea, and other conditions, yields a toxic degradation product, 4-epianhydrotetracycline (EATC). General Chapter 226 of the U.S. Pharmacopeia and National Formulary (USP-NF), referred to by monographs for epitetracycline and drug products containing tetracycline hydrochloride (TC-HCl), prescribes an antiquated assay for EATC impurity in TC.
Rapid Screening of Pesticides Using U-HPLC/MS
June 1st 2009The screening of pesticides, mycotoxins, and veterinary drugs is of great importance in regulated environments such as food or feed analysis. Due to some of the limitations of traditional triple quadrupole approaches (for example, targeted analyte detection, limited number of compounds, and unidentified unknown compounds), there is currently a trend towards use of full-scan MS data for the analysis of residue samples. Current screening approaches mainly rely on the use of ToF instruments coupled to U-HPLC delivering mass accuracy (~5 ppm) at a maximum resolution of <15,000. This can produce inaccurate mass measurements due the presence of unresolved background matrix interferences. In this work we show a full-scan MS screening approach with the Thermo Scientific Exactive mass spectrometer, a novel single-stage Orbitrapâ„¢ MS capable of providing precise mass accuracies at resolutions of up to 100,000 without the need for internal mass calibration.