Secondary parameters in the interface and mass analyzer can often have a major impact on sensitivity and reproducibility. This column considers how and when to consider optimizing these parameters through a study of the working principles of LC–MS analysis.
Entirely new sample preparation technologies continue to be introduced, mainly in the academic sector. Some of these technologies will undoubtedly stay in the academic laboratory. However, some new technologies may "cross the chasm" and eventually become a standard laboratory procedure. This instalment will examine some of those methods.