To obtain a better understanding of the challenges faced during method development and validation, the authors - in cooperation with LCGC - conducted a survey earlier this year. This column summarizes the results.
Scientists Jorn Chi Chung Yu, Garrett Lee Burleson, and colleagues in the Department of Forensic Science at Sam Houston State University in Texas have developed a new method for identifying gunshot residue without the presence of lead.
This article demystifies the qualification procedures and proposes a well designed, easy and simple set of experiments upon which to establish internal standard operating proceduresfor the complete qualification of HPLC instruments.
The authors discuss a preparative process using the principles of countercurrent chromatography. This process is faster, capable of loadings from milligrams to hundreds of grams, and uses robust equipment.