November 1999 | LCGC North America

Monoliths as Stationary Phases for Separating Biopolymers – Fourth-Generation Chromatography Sorbents

November 02, 1999

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Monoliths are chromatography sorbents cast into columns as a single continuous piece in contrast with regular chromatographic sorbents, which are packed as individual particles. The guest authors compare three such novel sorbents with a conventional particle-packed column.

A Coin-Toss Experiment, Part II – Limits of the Analogy

November 01, 1999

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In part I, Hinshaw described a simple experiment with coins to explain the statistical nature of separations and to impart a better understanding of the physical processes inside the column. In part II, he compares this coin-toss simulation with chromatographic columns and describes differences between the analogy and real-world situations.

Ion-Exchange Displacement Chromatography: Scale-Up and Displacer Clearance for Recombinant Human Brain-Derived Neurotrophic Factor

November 01, 1999

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Displacement chromatography is a viable alternative to elution chromatography, but the biotechnology industry has yet to fully exploit this technique. The authors present results of a pilot-scale ion-exchange displacement process developed for the removal of variants from recombitant human brain-derived neutrotrophic factor.

Cleaning Validation

November 01, 1999

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Krull and Swartz examine validating cleaning methods for pharmaceutical manufacturing equipment and look at general requirements and specific cleaning procedures, sampling types, and analytical methods.