Liquid Chromatography (LC/HPLC)

A research team has investigated the impact of hydrophilic/lipophilic balance (HLB) on the porogenic properties of non-ionic surfactants for chromatographic separation.

Researchers have recalibrated polyparameter linear free energy relationships (PP-LFERs) for low-density polyethylene (LDPE) passive samplers. By utilizing a robust regression method and reliable partition coefficients and solute descriptors, the recalibrated models exhibited improved predictive performance, accurately estimating LDPE-water and LDPE-air partition coefficients across a wide range of compounds.

Although closantel (CLO) is well-regarded as a curative antiparasitic drug, even low doses have been found to cause serious side effects in the human retina and central nervous system.

OECD-compliant amino acid derivative assay (ADRA) testing and method transfer to UHPLC conditions is presented.

Forsythiae fructus is a traditional Chinese medicinal herb that has been used to treat various ailments such as inflammation, fever, and infections.

LCGC was on the ground at HPLC 2023.

Examining the role that LC–MS/MS plays in metabolomics, and metabolomics’ growing importance in the field of disease biology.

This article details a recent experiment that demonstrates how nonspecific interactions can be prevented by using metal-free or low adsorption flow paths.

How to modernize an older pharmaceutical monograph from an HPLC column to an UHPLC column, along with calculated time and solvent savings.

Researchers have utilized computational fluid dynamics (CFD) simulations to optimize the design of membrane chromatography devices, enhancing fluid flow uniformity and separation efficiency.

A separation science team has developed a green and cost-effective method, called feather fiber-supported liquid extraction (FF-SLE), for determining synthetic antioxidants in edible oils.

Researchers have introduced a novel primary method utilizing gas chromatography–isotope dilution infrared spectrometry (GC–IDIR) for accurate peptide purity analysis.

Researchers have developed a robust method utilizing high-performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS) to accurately quantify amino acids in plasma, making it a valuable tool for clinical diagnostics and research in amino acid metabolism.

Enhancements in chromatographic performance were reviewed as were experimental concerns, both pointing toward using specific solvents to influence more sustainable industrial development.

Pop quiz – can you define resolution? Would you be surprised at the number of correct answers? Which one is the best for chromatographers? In this LCGC Blog, we look at the various ways resolution can be interpreted scientifically.

Levoglucosan, which is emitted from the combustion of cellulose and hemicelluloses, can be transported over a long range of time to ice caps, where it may indicate fire activity both regionally and globally.

Researchers have discovered that phospholipids form doubly charged lipid-metal ion complexes during electrospray ionization (ESI), providing valuable insights into the analysis of phospholipid structures.

Researchers have developed an ultrahigh-pressure liquid chromatography (UHPLC)—tandem mass spectrometry (MS/MS) method for the simultaneous determination of 10 aminoglycoside antibiotics in aquatic products.

Researchers have introduced MCnebula, a new framework for untargeted LC–MS/MS data analysis. MCnebula focuses on critical chemical classes and utilizes visualization techniques to streamline the analysis process, enabling efficient pathway analysis, biomarker discovery, and classification of unknown compounds beyond the limits of spectral libraries.

A new method has been developed for detecting and quantifying polycyclic aromatic hydrocarbons (PAHs) in intermediates used in plastics production. The method, based on liquid chromatography column switching, allows for the analysis of 18 PAHs and addresses the lack of regulatory oversight for PAH concentrations in plastic intermediates.

The team of researchers found that components with the same retention time can have different migration patterns, and discussed the different scenarios for decreasing column pressure resistance.

Researchers have developed a novel algorithm that combines multivariate curve resolution-alternating least-square (MCR-ALS) and Bayesian inference techniques to estimate confidence intervals for the quantitation of coeluted peaks in liquid chromatography-photodiode array detection.

In a lecture at HPLC 2023, Valérie Pichon of ESPCI Paris and Sorbonne University said these advances address limitations of more traditional extraction techniques.

In a presentation at HPLC 2023, Kevin Pagel of Freie Universität in Berlin, Germany described methods he has found most effective for better understanding the roles of glycans and glycoconjugates in biological processes.

At HPLC 2023, Paola Dugo held a lecture on how combining two-dimensional liquid chromatography (LC×LC) with mass spectrometry (MS) can help scientists analyze food samples.