A recent study analyzed the composition and metabolism of steroidal alkaloids in Solanum nigrum fruits of different maturities and identified steroidal glycoalkaloids and steroidal alkaloids, providing a foundation for further research on biosynthesis and discovery of new steroidal alkaloid anticancer drugs.
Proteotypic Peptides of Hairs Can Be Used for Species Identification Using Mass Spectrometry
Researchers Propose Use of Flavonoid Isoquercitrin As a Quality-Marker Candidate for Eucommiae Folium (Duzhongye) in Chinese Pharmacopoeia Using UHPLC–MS/MS Analysis
A new approach for identifying isomeric and isobaric metabolites has been developed using liquid chromatography (LC) with high-resolution ion mobility spectrometry (IMS) and cryogenic infrared (IR) spectroscopy, allowing for faster, more accurate, and cost-effective metabolomics workflows.
Researchers have developed a new approach to extract chloramphenicol (CAP) from milk and chicken samples using magnetic nanofluid-based deep eutectic solvents (DES), which was combined with high performance liquid chromatography-ultraviolet (HPLC-UV) analysis. The method was found to be low-cost, green, efficient, and effective in detecting even trace amounts of CAP.
Scientists have developed a magnetic ionic liquid hypercrosslinked polymer composite for use in magnetic solid-phase extraction (MSPE) of non-steroidal anti-inflammatory drugs (NSAIDs), which was coupled with high-performance liquid chromatography (HPLC) for the detection of trace amounts of NSAIDs in water and urine samples.
This article compares the performance of wide-pore silica monolithic, sub-2µm FPP, and SPP columns, addresses the question of whether 1000 Å or 400 Å SPP columns are more suitable for reversed-phase LC-type protein separations, and presents a kinetic performance comparison of different columns.
A micro-pillar array format for mapping the proteome of human stem cell-derived liver organoids using timsTOF–MS is presented.
This article highlights an advantage of porous graphitic carbon columns, that is their resistance at high temperatures, for the analysis of parabens in foodstuffs.
An introduction from guest editor, Gert Desmet from Vrije Universiteit Brussel, Belgium
Researchers have developed a new method for detecting pyrethroid pesticide residues in tobacco using Schiff base networks (SNWs) coated stir bar sorptive extraction (SBSE) coupled with high performance liquid chromatography-ultraviolet detection (HPLC-UV), which exhibited high extraction efficiency and good mechanical stability even when reused more than 50 times.
A new RPLC–MS method can map the substitution degree and composition of β-glucose monomers of cellulose ethers (CEs), providing detailed compositional distributions and constitutional isomer distributions, thereby identifying compositional variation between bio-stable and non-bio-stable CEs and unexpected methylations in EHEC samples.
The occurrence of convex-upward van Deemter curves is rare but not unusual in chiral liquid chromatography. In this work, this behaviour, experimentally observed for the more retained enantiomer of a chiral sulfoxide on a polysaccharide-based CSP, is explained.
High performance liquid chromatography–mass spectrometry and gas chromatography–mass spectrometry, along with numerous extraction methods, are helping to find organosulfur compounds in food more easily, providing researchers better information about the health benefits of these substances.
A recent trend in the design of LC instrumentation is the move towards miniaturized and portable systems.
What are the options available when adjusting selectivity (peak spacing or elution pattern) when dealing with relatively simple mixtures?
Exploring the impact of green chemistry on analytical chemistry and GC is the focus of this instalment of "GC Connections".
This article covers LC columns and accessories commercially released after Pittcon 2022 through the 2023 conference held in Philadelphia, USA.
A rapid and robust LC–MS/MS method for determining lactulose and rhamnose concentrations in blood plasma was used to determine intestinal permeability from blood plasma, which can help diagnose gastrointestinal diseases such as Crohn’s disease.
The combination of an untargeted approach using ultrahigh pressure liquid chromatography–quadrupole time-of-flight mass spectrometry (UHPLC–QTOF) and a targeted approach using UHPLC–tandem mass spectrometry (MS/MS) are presented as an ideal method for detecting per- and polyfluroalkyl substances (PFAS) in fast-food packaging.
Scouting gradients can simplify LC method development. Here’s what you need to get started using them.
Our annual review of new liquid chromatography columns and accessories, introduced at Pittcon and other events over the past 12 months.
Everyone is talking about sustainability, and organizations are creating sustainability programs. But what does green chemistry really mean, and how does it apply to gas chromatography?
This method has greater sensitivity and a shorter LC analysis time than the AOAC method. It also requires less instrument maintenance, enhances column lifetime by requiring a smaller sample volume, and reduces cost by replacing acetonitrile with methanol in the mobile phase.
Selective pressurized liquid extraction and multilayer solid‑phase extraction methods are described for high‑throughput plant, soil, and water sample preparations. Optimal analytical conditions are described for the improvement of detection sensitivities and coverage. A Source Supported Suspect Screening (4S) approach is described; phytotoxins detected in the source plant were used to improve the identification of phytotoxins in soil and water.
Researchers have shown the effectiveness of ion chromatography (IC) in identifying markers of forest fire emissions in rainfall samples, providing valuable insights into the signature of forest fire emissions and aiding in the development of models to predict and control air pollution resulting from these events.
A new study has reported the preparation, characterization, and chromatographic evaluation of a nickel oxide deposited silica stationary phase in HILIC, which exhibited multiple retention mechanisms and enhanced chromatographic selectivity for analytes.
A new approach combining high performance liquid chromatography–mass spectrometry with ultrafiltration, along with enzyme channel blocking and directional enrichment, has been found effective for screening natural histidine decarboxylase inhibitors in Radix Paeoniae alba, while reducing both false-positive and false-negative results.
