Researchers have developed a viable alternative to existing gas chromatography (GC) methods for glycerol determination in biodiesel using solid-phase extraction and high-performance liquid chromatography with a refractive index detector (SPE–HPLC-RI) (1).
Derived from the oils and fats of plants or animals, biodiesel is one of many alternatives to crude oil fuel sources. Considered a drop-in biofuel as it is compatible with existing diesel engines and distribution infrastructure, it is usually blended with petrodiesel, with 60 countries around the world mandating its addition. Countries such as the USA, Brazil, China, and Thailand all require petrodiesel to be blended with biodiesel in varying proportions, quite often hovering around the 10% biodiesel to 90% petrodiesel mark (2).
While biodiesel is compatible with modern diesel engines, the sole use of biodiesel as a fuel source is not possible without engine modifications, and in fact biodiesel contains a substance that can cause problems in car engines and in storage infrastructure (1). Glycerol, a co-product of the transesterification of oils and fats, needs to be monitored when blended with traditional petrodiesel. This is because during the blending process high concentrations of glycerol can separate out in the storage tanks. Furthermore, issues such as deposit formation, clogging of fuel injectors, and the production of harmful combustion products, such as aldehydes, are also related to high glycerol concentrations in biodiesel (3).
Currently a technique using GC monitors glycerol concentrations; however, this technique was developed for biodiesels made from canola oil and is not necessarily adequate for biodiesels made from other oils and fats. For example, in Brazil, around 70% of biodiesel is made from soybean oil, with the remaining 30% coming from animal tallow. Analysis with the current standard technique can often yield overlapping peaks, making analysis of biodiesel quality difficult. The great diversity of raw material used for biodiesel production presents many challenges for analytical chemists. Rising to that challenge, researchers aimed to develop a new method to detect and quantify glyercol in biodiesel using SPE and HPLC using a refractive index detector.
The resulting method proved capable of accurately quantifying glycerol in biodiesel samples made from raw materials containing different proportions of soybean oil and tallow. The new method offers a simple, quick, and effective method of determining free glycerol in biodiesel and a viable alternative to existing methods.
References
Inside the Laboratory: The Chromatography Laboratory at the University of Rouen
April 18th 2024In this edition of “Inside the Laboratory,” Pascal Cardinael and Valérie Agasse of the University of Rouen in Mont‑Saint-Aignan, France, discuss their laboratory’s work with miniaturizing gas chromatography (GC) columns and systems to improve on-site air analysis of volatile organic compounds (VOCs).
Inside the Laboratory: The McLean Group at Vanderbilt University
April 16th 2024In this edition of "Inside the Laboratory," John A. McLean, the dean of graduate education and research at the College of Arts & Science of Vanderbilt University, discusses his laboratory's recent work regarding ion mobility-mass spectrometry (IM-MS) and how it can be applied in various fields.