CESI–MS: An Essential Complement to Nano LC–ESI–MS for Complex Proteome Analysis
Liquid chromatography–electrospray ionization–mass spectrometry (LC–ESI–MS) is often used for the identification of proteins from complex peptide mixtures made from enzymatic digests of selected proteomes.
Liquid chromatography–electrospray ionization–mass spectrometry (LC–ESI–MS) is often used for the identification of proteins from complex peptide mixtures made from enzymatic digests of selected proteomes. However, these experiments only represent part of the proteome, as there are many peptides that are not ionized, retained or eluted under these reversed-phase LC–ESI conditions.
New Study Investigates Optimizing Extra-Column Band Broadening in Micro-flow Capillary LC
March 12th 2025Shimadzu Corporation and Vrije Universiteit Brussel researchers recently investigated how extra-column band broadening (ECBB) can be optimized in micro-flow capillary liquid chromatography.
