
|Articles|October 7, 2011
- The Column-10-07-2011
- Volume 7
- Issue 18
CESI–MS: An Essential Complement to Nano LC–ESI–MS for Complex Proteome Analysis
Liquid chromatography–electrospray ionization–mass spectrometry (LC–ESI–MS) is often used for the identification of proteins from complex peptide mixtures made from enzymatic digests of selected proteomes.
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Liquid chromatography–electrospray ionization–mass spectrometry (LC–ESI–MS) is often used for the identification of proteins from complex peptide mixtures made from enzymatic digests of selected proteomes. However, these experiments only represent part of the proteome, as there are many peptides that are not ionized, retained or eluted under these reversed-phase LC–ESI conditions.
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