The panelists introduce themselves and outline their practical experience and knowledge of oligonucleotides, setting the stage for an illuminating discussion on current challenges, solutions and innovations in this ten-part panel discussion series organised by LCGC International in collaboration with the Chromatographic Society (ChromSoc).
Michael Bartlett, Torgny Fornstedt and Mark Dickman elaborate on the possible reasons why ion-pair reversed-phase high performance liquid chromatography (IP-RP-HPLC) has risen to such prominence for the separation of oligonucleotides.
The panel discuss the role of ion-exchange chromatography (IEX) and hydrophilic interaction liquid chromatography IHILIC) in oligonucleotide analysis, and if these techniques could challenge ion-pair reversed-phase high performance liquid chromatography (IP-RP-HPLC) in the near future.
The panel explore the potential of multidimensional chromatography for the analysis of oligonucleotides.
Torgny Fornstedt describes how machine learning can work in practice for oligonucleotide analysis.
Mark Dickman shares his views on analyzing larger oligonucleotides, including mRNAS.
The panel explore the unique challenges associated with analysing double- stranded oligonucleotide products and oligonucleotides with higher-order structures.
The sensitivity and selectivity of high resolution mass spectrometry (HRMS) for oligonucleotide analysis is discussed.
The panel discuss the innovations in sample preparation that are being developed for oligonucleotide analysis.
Michael Bartlett, Torgny Fornstedt and Mark Dickman conclude the series with their expert insights into what the future of oligonucleotide analysis could involve.
