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Column developments in HPLC have benefited protein aqnd peptide separations in a number of ways. More silica based phases and column formats have become available, expanding the use of HPLC for the separation of proteins by different modes of chromatography as well as improving resolution and throughput. Hydrophobic interaction chromatography, immobilized metal affinity chromatography as well as ion Exchange and size exclusion are now practical analytical tools for proteins and peptides. New enrichment columns are benefiting proteomic research.