Application Notes: LC

mRNA coupled to EGFP (enhanced green fluorescent protein) is analyzed using a widepore YMC-Accura Triart Bio C4 RP column as well as a non-porous BioPro IEX QF column.

Explore how a systematic screening strategy allows chromatographers to rapidly identify ideal method conditions.

This white paper explores the theory behind solid-core particles and the advantages they offer for small molecule analysis.

This article discusses the impact and theory of column temperature and how it can be a powerful tool for fine-tuning a separation during method development.

An increased number of samples demands increased throughput. This article discusses ways to achieve high throughput analysis with LC–MS analysis methods.

In LC separations, the majority of analytes are ionizable, so controlling the state the analytes are in is critical. Discover how to harness pH for method development.

In this app note, a two-dimensional separation is conducted on an oxidative lignin depolymerization mixture using centrifugal partition chromatography.

The fast identification of tocopherols are important for various applications including free radical scavenging for human health and food stuff preservation.

The use of resistor tubing and 200 mM ammonium acetate in the mobile phase was implemented to measure loading capacity of a Biozen 1.8 μm dSEC-2 column.

Protein standards were explored with known hydrodynamic radii, showing how this can be used to determine ideal size exclusion conditions for optimal method performance.

Using standard phosphate containing buffers with different percentages of isopropanol for aggregate analysis of an antibody-drug conjugate (ADC), Kadcyla

Temperature, gradient program, and flow rates are explored and optimized for intact mass applications for mAbs when using Biozen WidePore C4 column with bioTi hardware.

The hydrophilic nature of the Biozen dSEC-2 stationary phase shows favorable aggregate profiles for forced degradation work under high salt conditions.

A robust and sensitive HPLC method with multi-wavelength UV–vis detector for the analysis of 2-phenoxyethanol and seven parabens in cosmetic and personal care products.

Molnupiravir, which is a prodrug, and its active agent β-d-N4-hydroxycytidine can be separated by using a YMC-Triart C18 UHPLC column and a MS-compatible mobile phase.

This work introduces the analysis of iso-α-acids, α-acids, and humulinones in beer in reference to EBC 9.47. IBU was also measured with a UV–vis spectrophotometer.

In this app note, xanthohumol, isoxanthohumol, humulinones, iso-α-acids, α-acids, and β-acids are simultaneously analyzed by high performance liquid chromatography.

This article introduces the high-speed analysis of α-acids and β-acids in hops in reference to EBC 7.7 and ASBC Hops-14.

Volatile sulfur compounds were analyzed in commercially available beer samples using GC with headspace sampler and sulfur chemiluminescence detection.

Shimadzu provides total solutions for beer analysis from farm to stein and service support to keep up with changing market demands.

This application describes the analysis of organic acids in beer by ion-exclusion chromatography and postcolumn pH-buffering conductivity detection.

This application note covers high-resolution oligonucleotide analysis using FlexFire’s new Wide Pore (WP) C18 1.6 µm column.

YMC-Triart Bio C4 is used to successfully analyze monoclonal antibodies in reversed-phase mode in this robust application.

This application note demonstrates the use of YMC-Triart C18 ExRS for UHPLC potency analysis of 11 common cannabinoid compounds.

This application note explores when to use each of YMC’s two HIC columns by comparing retention behaviors of BioPro HIC HT and BioPro HIC BF.

FFF combined with NTA can be used in the development of therapeutics for biological samples for the optimization process in clinical trials under in vivo conditions.

This brief communication highlights the assessment of Hamilton PRP-3 HPLC column attributes when analyzing biologically active peptides.

This case study demonstrates how method modernization is less difficult than the common perception, and good practices can be put in place to streamline the process.  

Analyzing crops for beneficial and harmful compounds is an indispensable tool for assessing the viability of various agricultural crops for consumption.