Application Notes: LC

Learn how a single method was developed for simultaneous determination of two active ingredients, replacing several separate methods for the analysis of assays and organic impurities.

Find out how the Alliance iS HPLC System makes method migration more straightforward with usability features that limit analyst error and improve system reliability.

Pharma QC labs face growing demands to do more with less. With capabilities developed specifically for the QC lab, the Alliance iS HPLC System will quickly become your favorite ally.

Learn how to generate quality data that meets the regulatory requirements of the U.S. Pharmacopeia Chapter <621> with the Alliance iS HPLC System.

See how the Alliance iS HPLC System’s tool-free fittings and improved wash function can eliminate volumetric carryover and reduce adsorptive carryover, respectively.

Peptide analysis in the GI tract shows links to endocrine, cardiac, and nervous systems. See how Hamilton Company isolates GPCR binding peptides with its PRP-3 column.

Find out how the Alliance iS HPLC System makes method migration more straightforward with usability features that limit analyst error and improve system reliability.

See how the Alliance iS HPLC System’s tool-free fittings and improved wash function can eliminate volumetric carryover and reduce adsorptive carryover, respectively.

This application shows how Shodex HILICpak columns can separate high-molecular weight oligosaccharides that proved difficult with preexisting HILIC columns.

This application note shows how using the Shodex HILICpak VC-50 2D column with LC–MS provides highly sensitive analyses of various low molecular weight cationic compounds.

Triazines in water supplies cause endocrine disruption in reproduction of some animals. See how Hamilton's HPLC columns can separate seven federally approved triazines.

Analyzing crops for beneficial and harmful compounds is an indispensable tool for assessing the viability of various agricultural crops for consumption.

Honey has medicinal and healing properties, but it often contains refined sugars to extend supply. See how Hamilton's HC-75 column exposed Nevada honey adulteration.

This application note highlights the assessment of Hamilton PRP-3 HPLC column attributes when analyzing biologically active peptides.

This application note explains how Hamilton Company's PRP-C18 method offers a fast, simple solution for identifying vitamin K congeners.

This application note describes a method to confirm the seven most common flavonoids found in cannabis using the PRP-1 column.

The Focus LC® separated PAHs in ~12 and ~15 min, with separate methods on short capillary columns packed with 1.7 µm monomeric and 3.0 diameter polymeric C18 particles.

The separation of five test proteins in the range of 5.8 to 45 kDa is shown using reversed phase (RP) conditions.

The Hamilton PRP-C18 reversed-phase column offers good selectivity and peak shape when detecting the three major components of THC metabolites in urine.

Real time determination of the presence of MDA in an aviation turbine fuel sample at installation level or in a field environment outside of a laboratory.

A point-of-care LC system that is compact, reliable, and easy to use. The Axcend Focus LC is efficient, allowing for informed decisions at the time of need.

Repeatable analyses of API dissolution. An Extra Strength Excedrin® analgesic tablet was dissolved in a 200 mL solution (97% water; 3% ACN; and <0.1% TFA).

Portable HPLC to detect and separate 5 cannabinoids present in 50 mg of a hemp-variant of cannabis flowers and 12 additional cannabinoids present in a Restek standard.

Although the majority of benzodiazepines metabolize to a single compound, oxazepam, other metabolites can be detected by liquid chromatography.

In this study, a generic, 5-min linear gradient was used to separate six basic drug compounds on a short (50 mm) PRP-C18 column.

The Raffinose Family of Oligosaccharides (RFO) is comprised of low molecular weight non-reducing sugars. Analysis of these compounds is not easily done unless using HC-75.

Paclitaxel originates from the Pacific Yew tree. The isolation of paclitaxel from its major impurities is shown with the use of Hamilton’s PRP-1 (5 µm) HPLC column.

The fast simple method developed by Hamilton Company using the PRP-C18 column allows for simple, efficient identification of the whole vitamin series (K2, K3, and K4).