Application Notes: LC

The separation of five test proteins in the range of 5.8 to 45 kDa is shown using reversed phase (RP) conditions.

The Hamilton PRP-C18 reversed-phase column offers good selectivity and peak shape when detecting the three major components of THC metabolites in urine.

Real time determination of the presence of MDA in an aviation turbine fuel sample at installation level or in a field environment outside of a laboratory.

A point-of-care LC system that is compact, reliable, and easy to use. The Axcend Focus LC is efficient, allowing for informed decisions at the time of need.

Repeatable analyses of API dissolution. An Extra Strength Excedrin® analgesic tablet was dissolved in a 200 mL solution (97% water; 3% ACN; and <0.1% TFA).

Portable HPLC to detect and separate 5 cannabinoids present in 50 mg of a hemp-variant of cannabis flowers and 12 additional cannabinoids present in a Restek standard.

Although the majority of benzodiazepines metabolize to a single compound, oxazepam, other metabolites can be detected by liquid chromatography.

In this study, a generic, 5-min linear gradient was used to separate six basic drug compounds on a short (50 mm) PRP-C18 column.

The Raffinose Family of Oligosaccharides (RFO) is comprised of low molecular weight non-reducing sugars. Analysis of these compounds is not easily done unless using HC-75.

The fast simple method developed by Hamilton Company using the PRP-C18 column allows for simple, efficient identification of the whole vitamin series (K2, K3, and K4).

A Hamilton PRP-C18 (5 μm) HPLC column was used for purification of three oligonucleotides. Analysis of a 21-mer, 22-mer, and 23-mer of similar structure was evaluated.

The primary nucleosides encompass a broad array of biological functions associated with RNA, particularly after configuration into multidimensional conformations.

This application note describes a 2D-LC workflow for Protein A purification of mAbs, followed by online reduction and MS analysis of mAb subunits.

A separation of parabens is performed on a HALO 90 Å LPH-C18 column under low pH (pH 1) and high temperature conditions compared to a standard C18 SPP column.

A low pH compatible, 90 Å, superficially porous particle C18 phase is introduced as an addition to the chromatographer’s toolbox.

The natural products of the cannabis plant can number up to 100, but the scientific community is concerned with a smaller portion of around 10 to 20 compounds.

A low pH compatible, 90 Å, superficially porous particle C18 phase is introduced as an addition to the chromatographer’s toolbox.

A HALO® LPH-C18 column is used to separate a mixture of 18 cannabinoids, showing fast results and high resolution within critical pairs.

The fast identification of tocopherols is important for various applications including free radical scavenging for human health and food stuff preservation.

mRNA coupled to EGFP (enhanced green fluorescent protein) is analyzed using a widepore YMC-Accura Triart Bio C4 RP column as well as a non-porous BioPro IEX QF column.

Explore how a systematic screening strategy allows chromatographers to rapidly identify ideal method conditions.

This white paper explores the theory behind solid-core particles and the advantages they offer for small molecule analysis.

This article discusses the impact and theory of column temperature and how it can be a powerful tool for fine-tuning a separation during method development.

An increased number of samples demands increased throughput. This article discusses ways to achieve high throughput analysis with LC–MS analysis methods.

In LC separations, the majority of analytes are ionizable, so controlling the state the analytes are in is critical. Discover how to harness pH for method development.

In this app note, a two-dimensional separation is conducted on an oxidative lignin depolymerization mixture using centrifugal partition chromatography.

The fast identification of tocopherols are important for various applications including free radical scavenging for human health and food stuff preservation.

The use of resistor tubing and 200 mM ammonium acetate in the mobile phase was implemented to measure loading capacity of a Biozen 1.8 μm dSEC-2 column.