Application Notes: LC

In this study, a generic, 5-min linear gradient was used to separate six basic drug compounds on a short (50 mm) PRP-C18 column.

Isolation of six common allergens can be achieved by utilizing reversed-phase HPLC using an alkaline eluent.

The identification benzodiazepine metabolites can be highly valuable in detecting abuse. Metabolites of oxazepam are found containing both hydroxyl and glucuronides.

This brief communication highlights the assessment of Hamilton PRP-3 HPLC column attributes when analyzing biologically active peptides.

This application note demonstrates the performance of multi-detector asymmetrical flow field-flow fractionation for the characterization of macromolecules in beer.

A Hamilton PRP-C18 (5 μm) HPLC column was used for purifying three oligonucleotides. Analysis of a 21mer, 22mer, and 23mer of similar structure were evaluated.

Sulfide compounds found in garlic oils are used in therapeutics and prophylaxis of certain cancers, and are prevalent in cell health.

Identification and quantification of the Raffinose Family of Oligosaccharides allows a more thorough understanding of an individual’s gut health.

Garlic has been found to exhibit a multitude of proliferative health benefits in the realm of cardiac events.

Isolation of capsaicin for medicinal uses is easily achieved with the PRP-C18 RP-HPLC resin.

Excellent separation and chemical stability in oligonucleotide isolation is observed. The C18 column provides loading capacities and reliability needed for repeat use.

Identification of the five most common saccharides found in packaged foods, lactose, maltose, sucrose, fructose, and glucose, is easily resolved on the RCX-30 column.

GRPC peptides are found in the entire body. Isolation of seven classic GRPC peptides initiating in the GI tract could implicate disease.

Hydrophilic Interaction Liquid Chromatography (HILIC) can provide better separation for oligosaccharide analysis than Size Exclusion Chromatography (SEC) mode does.

This application demonstrated the effectiveness of Shodex™ HILICpak™ VG-50 4E with its HILIC mode for the analysis of various sugars found in Japanese sake.

mRNA coupled to EGFP (enhanced green fluorescent protein) is analyzed using a widepore YMC-Accura Triart Bio C4 RP column as well as a non-porous BioPro IEX QF column.

Different oligonucleotide mixtures were analyzed using the bioinert coated column YMC-Accura Triart Diol-HILIC in comparison to the conventional stainless steel column.

Four phosphopeptides are analyzed using a bioinert coated YMC-Accura Triart C18 column. The column provides high intensities, recoveries, and great peak shapes.

This application note demonstrates improved performance when separating polar compounds using Agilent's InfinityLab Poroshell 120 Aq-C18 column

This application note demonstrates the analysis of ten vitamin B compounds plus ascorbic acide and caffeine using Agilent's InfinityLab Poroshell 120 Aq-C18 column.

Four phosphopeptides are analyzed using a bioinert coated YMC-Accura Triart C18 column, providing high intensities, recoveries, and great peak shapes.

This application note describes online monitoring of a chemical reaction that produces a byproduct, and presents a fully automated sampling workflow.

Learn how a single method was developed for simultaneous determination of two active ingredients, replacing several separate methods for the analysis of assays and organic impurities.

Find out how the Alliance iS HPLC System makes method migration more straightforward with usability features that limit analyst error and improve system reliability.

Pharma QC labs face growing demands to do more with less. With capabilities developed specifically for the QC lab, the Alliance iS HPLC System will quickly become your favorite ally.

Learn how to generate quality data that meets the regulatory requirements of the U.S. Pharmacopeia Chapter <621> with the Alliance iS HPLC System.

See how the Alliance iS HPLC System’s tool-free fittings and improved wash function can eliminate volumetric carryover and reduce adsorptive carryover, respectively.

Peptide analysis in the GI tract shows links to endocrine, cardiac, and nervous systems. See how Hamilton Company isolates GPCR binding peptides with its PRP-3 column.

Find out how the Alliance iS HPLC System makes method migration more straightforward with usability features that limit analyst error and improve system reliability.

See how the Alliance iS HPLC System’s tool-free fittings and improved wash function can eliminate volumetric carryover and reduce adsorptive carryover, respectively.