Application Notes: LC

Using standard phosphate containing buffers with different percentages of isopropanol for aggregate analysis of an antibody-drug conjugate (ADC), Kadcyla

Temperature, gradient program, and flow rates are explored and optimized for intact mass applications for mAbs when using Biozen WidePore C4 column with bioTi hardware.

The hydrophilic nature of the Biozen dSEC-2 stationary phase shows favorable aggregate profiles for forced degradation work under high salt conditions.

A robust and sensitive HPLC method with multi-wavelength UV–vis detector for the analysis of 2-phenoxyethanol and seven parabens in cosmetic and personal care products.

Molnupiravir, which is a prodrug, and its active agent β-d-N4-hydroxycytidine can be separated by using a YMC-Triart C18 UHPLC column and a MS-compatible mobile phase.

This work introduces the analysis of iso-α-acids, α-acids, and humulinones in beer in reference to EBC 9.47. IBU was also measured with a UV–vis spectrophotometer.

In this app note, xanthohumol, isoxanthohumol, humulinones, iso-α-acids, α-acids, and β-acids are simultaneously analyzed by high performance liquid chromatography.

This article introduces the high-speed analysis of α-acids and β-acids in hops in reference to EBC 7.7 and ASBC Hops-14.

Volatile sulfur compounds were analyzed in commercially available beer samples using GC with headspace sampler and sulfur chemiluminescence detection.

This application describes the analysis of organic acids in beer by ion-exclusion chromatography and postcolumn pH-buffering conductivity detection.

This application note covers high-resolution oligonucleotide analysis using FlexFire’s new Wide Pore (WP) C18 1.6 µm column.

YMC-Triart Bio C4 is used to successfully analyze monoclonal antibodies in reversed-phase mode in this robust application.

This application note demonstrates the use of YMC-Triart C18 ExRS for UHPLC potency analysis of 11 common cannabinoid compounds.

This application note explores when to use each of YMC’s two HIC columns by comparing retention behaviors of BioPro HIC HT and BioPro HIC BF.

FFF combined with NTA can be used in the development of therapeutics for biological samples for the optimization process in clinical trials under in vivo conditions.

This brief communication highlights the assessment of Hamilton PRP-3 HPLC column attributes when analyzing biologically active peptides.

This case study demonstrates how method modernization is less difficult than the common perception, and good practices can be put in place to streamline the process.  

Analyzing crops for beneficial and harmful compounds is an indispensable tool for assessing the viability of various agricultural crops for consumption.

This application note covers oligonucleotide analysis using FlexFire’s new Wide Pore (WP) C18 1.6 µm column.

Plasticizers added to water bottles to add stability to the bottles can be unknowingly extracted in drinks leading to possible endocrine interactions. 

This application note demonstrates the performance of the miniLC LC–UV analyzer for the analysis of catechins and epicatechins in teas.

This app note shows how CPC can produce a high-quality standard of [6]-gingerol without the use of silica-based chromatography methods, such as FLASH and prep HPLC.

A rapid and simple LC method for the analysis of six common VOC metabolites in urine for monitoring occupational exposure to VOCs.

We examined FlexFire columns and their analytical conditions using the synthesized miRNA samples. For this study, we applied inert UHPLC columns to prevent sample loss.

This application details how the FlexFire mAb-RP column allows for accurate analysis of a wide range of molecular sizes.

Large viral vectors are characterized rapidly using dynamic light scattering and in-depth by multi-angle light scattering in combination with field-flow fractionation.

This bibliography is a guide to the scope of Vanquish HPLC and UHPLC usage in literature. Publications have been categorized based on either target analyte or field of application.