Application Notes: LC

BioCore HIC column family separate protein-based biotherapeutics and characterizing their critical quality attributes, including DAR and drug distribution in ADCs

High Throughput Quality Control (HTQC) is essential for laboratories analyzing dozens, hundreds, or even thousands of samples daily. This application note explores the critical elements necessary to achieve speed, reliability, and automation in high-throughput analysis. Find out how integrating advanced techniques—focused on increasing speed, sample throughput, and parallel processing—results in a robust system capable of improving your daily sample volume

Identification of polyphenolic catechin compounds for health and industrial quantification and quality control of tea products.

A novel HIC column featuring innovative particle technology is benchmarked for separation of high DAR species ADCs, against a reference column with conventional chemistry.

With this AEX method a mix of six oligonucleotides can be separated in under two minutes.

This step-by-step method development study for a GLP-1 receptor agonist provides a robust and precise analytical workflow for purity testing of tirzepatide.

This note presents platform methods to characterize ADC using BioCore columns: SEC for aggregate determination, HIC/RP for DAR assesment, CEX for charged variant analysis.

HPLC is the method of choice for enhancing the purity of synthesized peptides. Here, a systematic and efficient workflow for scaling up peptide purification is shown.

Polysome profiling is a method for analysing the translational status of cells. This AppNote describes a simple FPLC system for polysome fractionation.

The diterpenoid, paclitaxel, which was identified as a potent chemotherapy agent for breast and ovarian cancer originates from the Pacific Yew tree. The isolation of paclitaxel from its major impurities is shown with the use of Hamilton’s PRP-1 (5 µm) HPLC column.

The identification benzodiazepine metabolites can be highly valuable in detecting abuse. Metabolites of oxazepam are found containing both hydroxyl and glucuronides.

Plasticizers added to water bottles to add stability to the bottles can be unknowingly extracted in drinks leading to possible endocrine interactions.

The fast identification of tocopherols are important for various applications including free radical scavenging for human health and food stuff preservation.

Isolation of capsaicin for medicinal uses is easily achieved with the PRP-C18 RP-HPLC resin.

Discover how the HC-75 series uses a ligand exchange mechanism in order to interact more or less strongly with the analytes of interest.

This brief communitation hightlights the assessment of Hamilton PRP-3 HPLC column attributes when analyzing biologically active peptides.

This application note introduces our simple and effective KNAUER HPLC Method Converter for modernising analytical methods according to the allowed USP 621 variations.

Application Notebook highlighting Liquid Chromatography techniques commonly used for monitoring Critical Quality Attributes (CQAs) in Bioprocessing Applications

Strategies to screen and develop a preparative HPLC method and its up-scaling for purification of CBD from Cannabis extract

The diterpenoid, paclitaxel, which was identified as a potent chemotherapy agent for breast and ovarian cancer originates from the Pacific Yew tree. The isolation of paclitaxel from its major impurities is shown with the use of Hamilton’s PRP-1 (5 µm) HPLC column.

Garlic has been found to exhibit a multitude of proliferative health benefits in the realm of cardiac events.

A reversed-phase HPLC method was developed to separate five steroid hormones, with partition coefficients ranging from 1.47 (cortisone) to 4.5 (pregnenolone).

Sulfide compounds found in garlic oils are used in therapeutics and prophylaxis of certain cancers, and are prevalent in cell health.

Identification of the five most common saccharides found in packaged foods, lactose, maltose, sucrose, fructose, and glucose, is easily resolved on the RCX-30 column.

The Raffinose Family of Oligosaccharides (RFO) is comprised of low molecular weight non-reducing sugars. Analysis of these compounds is not easily done unless using HC-75.

Isolation of six common allergens can be achieved by utilizing reversed-phase HPLC using an alkaline eluent.

With the growing utility of the K vitamin series of molecules, a need appears relevant for both food labeling of constituents and for research based separation of the natural and synthetic compounds of vitamin K. The fast simple method developed by Hamilton Company using the PRP-C18 column allows for simple efficient identification of the whole vitamin series (K2, K3, and K4).

Experience high recoveries from the first injection! YMC Accura BioPro IEX SF columns provide excellent peak shapes for the ion-exchange analysis of mAbs.