Application Notes: LC

Achieve robust tirzepatide analysis through a structured RP method enabling consistent workflows and easy transfer to other GLP-1 receptor agonists.

Accelerate a novel approach for mRNA characterization with innovative 2D-LC fingerprinting using a bioinert-coated YMC Accura Bio Pro IEX QF column.

Achieve precise purity analysis of peptide-oligonucleotide conjugates using an optimized IP-RP method with a bioinert YMC Accura Triart Bio C18 column for reliable QC.

Improved analysis of tryptophan and its metabolites using a bioinert YMC Accura Triart C18 column enabling reliable results in complex biological matrices.

Meet labeling demands with confidence. Hamilton’s RCX-30 column enables accurate quantification of five key sugars for compliant, high-throughput food and beverage QC.

Hamilton's PRP-Z2 solves analytical challenges of EPA-regulated diquat and paraquat separation through enhanced design, enabling fast, robust analysis with improved throughput and reduced solvent use.

Fast, accurate sugar analysis for food quality and labeling. Hamilton's HC-75 Ca++ column separates 8 sweeteners in under 15 minutes using only DI water.

PRP-Z2 columns enable high-resolution oligonucleotide separation with robust performance, reduced salt use, and scalability for research and regulated workflows.

Optimize fermentation faster and cheaper. Discover how Hamilton’s HC75 column speeds analysis, sharpens resolution, and boosts quality control in brewing and biofuels.

Hamilton's PRP-Z2 stationary phase enables fast, robust separation of diquat and paraquat herbicides under EPA 549.2 and EPA 539 conditions.

Highlights amino acid analysis of rice and millets using a high-speed amino acid analyzer with a protein hydrolysate method, supporting nutritional characterization.

Since selenium is found in both food, water, and soil, its relative steady state can be a little challenging owing to its different oxidation states.

Identification of polyphenolic catechin compounds for health and industrial quantification and quality control of tea products.

This report shows how Hitachi’s Amino Acid Analyzer delivers high-speed, high-resolution analysis of HCl-hydrolyzed cat, dog, bird, and goldfish pet food samples

The KNAUER Sepapure oliGO column is a good alternative to the commonly used competitor column for oligonucleotide analysis.

A novel HIC column featuring innovative particle technology is benchmarked for separation of high DAR species ADCs, against a reference column with conventional chemistry.

BioCore HIC column family separate protein-based biotherapeutics and characterizing their critical quality attributes, including DAR and drug distribution in ADCs

High Throughput Quality Control (HTQC) is essential for laboratories analyzing dozens, hundreds, or even thousands of samples daily. This application note explores the critical elements necessary to achieve speed, reliability, and automation in high-throughput analysis. Find out how integrating advanced techniques—focused on increasing speed, sample throughput, and parallel processing—results in a robust system capable of improving your daily sample volume

Identification of polyphenolic catechin compounds for health and industrial quantification and quality control of tea products.

A novel HIC column featuring innovative particle technology is benchmarked for separation of high DAR species ADCs, against a reference column with conventional chemistry.

With this AEX method a mix of six oligonucleotides can be separated in under two minutes.

This step-by-step method development study for a GLP-1 receptor agonist provides a robust and precise analytical workflow for purity testing of tirzepatide.

This note presents platform methods to characterize ADC using BioCore columns: SEC for aggregate determination, HIC/RP for DAR assesment, CEX for charged variant analysis.

HPLC is the method of choice for enhancing the purity of synthesized peptides. Here, a systematic and efficient workflow for scaling up peptide purification is shown.

Polysome profiling is a method for analysing the translational status of cells. This AppNote describes a simple FPLC system for polysome fractionation.

Organic solvent, pH, salt concentration, and gradient mixing rates influence HIC separations. Optimizing these factors allows for effective separation of mAbs and ADCs.

The diterpenoid, paclitaxel, which was identified as a potent chemotherapy agent for breast and ovarian cancer originates from the Pacific Yew tree. The isolation of paclitaxel from its major impurities is shown with the use of Hamilton’s PRP-1 (5 µm) HPLC column.