Application Notes: LC
Merck KGaA, Darmstadt, Germany: BIOshell™ 1.5 mm for greener HPLC
This application notes showcases greener HPLC with less solvent using smaller inner diameter columns while maintaining the quality of separation
Millipore Sigma: BIOshell™ 1.5 mm for greener HPLC
Merck KGaA, Darmstadt, Germany: Ascentis® Express for greener HPLC
This application notes showcases greener HPLC with less solvent using columns of shorter length and smaller inner diameter while maintaining the quality of separation
Millipore Sigma: Ascentis® Express for greener HPLC
Enhanced separation of plasmid isoforms via HIC
Discover the outstanding separation of three plasmid DNA isoforms, linear, open circular and supercoiled in HIC mode using YMC’s BioPro HIC BF column.
Understanding the importance of pump performance
Real time monitoring of flow using a non-invasive flowmeter offers a new diagnostic tool for measuring performance & troubleshooting problems with pumps serving HPLC systems
Full-empty analysis of AAVs
This innovative AEX method replaces toxic TMAC with non-toxic choline-Cl, ensuring safety and efficiency in AAV capsid analysis, applicable across multiple AAV serotypes.
Benefits of UHPLC
This application note shows the performance of the KNAUER AZURA UHPLC system to speed up your method and increase throughput preserving resolution.
Realtime Monitoring of HPLC Pump Performance
The described method of continuous flow rate monitoring provides real-time validation of total HPLC system performance and as such can improve reliability of results.
Efficient Method Development of Oligonucleotides
This article describes how to achieve optimal separation of oligonucleotides and related impurities via a dedicated method development software package.
Chiral Columns for Oligonucleotides Analysis (Nov 2023)
Accurate purity assessment of oligonucleotide mixtures can be challenging due to their complexity. Daicel offers several solutions that provide unique selectivity.
Determination of Adenine and Guanine Nucleotides
The measurement of adenosine nucleotides represents important diagnostic measures concerning metabolic standing for the determination of various pathological states.
Separation of Fat-Soluble Vitamin K Congeners
The coagulation benefits of vitamin K1 are well known, however, the other congeners of the vitamin series are not as common (K2, K3, and K4), but equally contribute to a healthy disposition.
Poisoned by Plastic: Analysis of BPA (Nov 2023)
Plasticizers added to water bottles to add stability to the bottles can be unknowingly extracted in drinks leading to possible endocrine interactions.
Legumes – Blessing and Curse (Nov 2023)
Discover how the HC-75 series uses a ligand exchange mechanism in order to interact more or less strongly with the analytes of interest.
Oligonucleotide Purification by Reversed-Phase Ion Pairing (Nov 2023)
Hamilton's PRP-C18 column gave good separation with various gradient profiles and under various particle sizes. The excellent loading capacity of the column make this stationary phase desirable.
Separating 12 Nucleosides on Hamilton Column
Modified nucleosides, either natural or synthetic, often provide RNA with distinctive properties useful in diagnostics or therapeutics.
Cannabis Potency by LC-DAD
This demonstrates how to perform cannabis potency analysis using HPLC-UV, displaying the advantages of this technique for analyzing acidic cannabinoids.
Comparing HPLC flow rate measurement techniques
Accurate determination of flow rate is an increasingly favoured method of assessing or validating the performance of a pump serving a liquid chromatography system.
Separation of Fat-Soluble Vitamin K Congeners in Under 6 Minutes
A fast, simple method using the PRP-C18 column allows for simple, efficient identification of the natural and synthetic compounds of vitamin K (K2, K3, and K4).
Arsenic Speciation on Hamilton PRP-X100 (Oct 2023)
Contamination of drinking water and food sources is of great concern. Isolation and quantification of the various arsenic states is identified.
Isolation of Capsaicin for Therapeutic Treatments (Oct 2023)
Isolation of capsaicin for medicinal uses is easily achieved with the PRP-C18 RP-HPLC resin.
Isoflavones and Mycotoxins in Soy Beans (Oct 2023)
Analyzing crops for beneficial and harmful compounds is an indispensable tool for assessing the viability of various agricultural crops for consumption.
Determination of Flavonoids in Hemp (Oct 2023)
Flavonoids in cannabis, fruits and vegetables protect plants. Hamilton has developed a method to confirm the 7 most common flavonoids in cannabis with the PRP-1 column.
Separation of Halo Acetic Acids by Ion Exclusion (Oct 2023)
Accurate detection of HAAs is paramount for prevention of overoxidation while still managing enough water sanitation to eliminate water-borne pathogens.
Peptide Mapping of AAV Capsid Proteins
In this application note, we profile capsid protein peptides from AAV5 and AAV9, digested with trypsin using a Biozen™ Peptide XB-C18 reversed phase column.
mRNA Cap Purity Analysis
We present an LC-HRMS approach to distinguishing a pool of multiple caps and their relative integrity of digested mRNA oligonucleotide strands of 1250 nucleotides.
Signature Peptide ID By HRMS
mRNA Poly A Tail Degradation Detection
Intact Identification of AAV Capsid Proteins
Review profiling of denatured and dissociated capsid proteins from AAV5 and AAV9, via analysis with LC-MS using a Biozen™ Intact XB-C8 reversed phase column.