Application Notes: Pharmaceuticals

Chemical modification of therapeutic proteins in order to enhance their biological activity is of increasing interest.

The Chirosil RCA(+) and SCA(–) chiral stationary phases (CSPs) are specialized chiral phases very effective in separating various natural and unnatural amino acids as well as compounds containing primary amines.

An extension of ASTM D7169 is shown using new Zebron ZB-1XT SimDist metal GC columns.

In developing the Atomx automated sample prep system, Teledyne Tekmar integrated both a P&T concentrator and a multi-matrix autosampler into a single platform system that can be used in the analysis of soil, water, and soils requiring methanol extraction.

Illicit cocaine, amphetamine, methamphetamine, and ecstasy (MDMA) are often encountered in the form of salts and identification of the specific counter ion present may provide valuable information about the source of production and the manufacturing pathway.

Hydroxyethylstarches (HES) are used increasingly as plasma expanders in medical applications.

Coffees have aroma and taste based on where the beans are grown, roasting techniques, and flavor additives.

This note examines the benefits of using flash or HPLC for compound purification. Results show that flash is a viable alternative to HPLC.

Burning mosquito coils to repel these pests is common in many countries; however the emission of various, potentially toxic volatile organic compounds (VOCs) is cause for concern.

Levoglucosan is a combustion product of cellulose and, as such, widely used as a unique tracer for biomass burning sources in atmospheric aerosols.

Agilent Poroshell 120, a superficially porous particle column, offers resolution and speed similar to columns packed with 1.8-µm totally porous particles, without generating high back pressure.

Gel filtration chromatography is the primary method used to analyze the amount of aggregate and dimer present in a therapeutic protein sample.

This paper describes the use of the AB SCIEX TripleTOFTM 5600 LC–MS-MS system for targeted and nontargeted screening for pesticide residue in food samples.

Fast, flexible platforms for peptide quantification are needed, particularly for a discovery setting.

The optical isomers of flavanone have been separated using the EpitomizeTM CSP-1 series of chiral stationary phases.

This article reports a new sample injection mechanism for an autosampler that directly transfers a sample to column without passing it through any flow-changing device, and hence minimizes a delay volume and reduces a carryover signal to the extreme level.

In this application note we present an online SPE-LC method for the determination of trace-level endocrine disruptive compounds (EDCs) in seawater using the new SolEx HRP cartridge as the solid-phase extraction (SPE) phase.

A sample of ambient cabin air from a commercial airliner was collected by stir bar sorption extraction (SBSE) and analyzed by thermal desorption GCxGC-TOFMS.

The new MAbPacTM size exclusion (SEC) column is based on high-purity, spherical, porous (300 Å), 5 µm silica covalently modified with a proprietary diol hydrophilic layer.

Site-specific analysis of protein glycosylation has become a very important issue in pharmaceutical/biotech industries, requiring specific analytical tools and methodologies.

Urine is often used to analyze drugs of abuse because it is easy to collect and is available in copious amounts.

Aflatoxins occur naturally in peanuts, cottonseed, corn, and dried chili pepper, as well as many mixed or processed foods and feeds.

Method development in reversed-phase liquid chromatography (RPLC) can be challenging for structurally similar compounds.

Electrochemical detection (ECD) is both selective and extremely sensitive. However, relatively few compounds are electrochemically active, therefore limiting its application.

Complete high resolution cross-fractionation of Polyolefins in the molar mass and composition dimensions is achieved with a new benchtop CFC analytical instrument.

Methods: Vials and closures were exposed to acetonitrile for 2 h and subsequently analysed by LC-UV, LC–MS, and GC–MS to characterize the interferences.

Whisky is a complex and variable material, the quality of which is affected by the raw materials and processing.

Gel-permeation chromatography (GPC) is a highly effective postextraction cleanup method for removing high molecular-weight interferences such as lipids, proteins, and pigments from sample extracts prior to analysis.