Application Notes: Size-Exclusion Chromatography (SEC)
Characterizing Vaccines with Light Scattering
Learn how light scattering characterizes multiple biophysical attributes of different vaccine modalities in support of discovery, development, manufacture, and QC.
Analysis of Hyaluronic Acid by SEC-MALS-IV
Comprehensive physicochemical characterization of biopolymers is accomplished by adding multi-angle light scattering and differential viscometry to standard SEC.
Polymer Branching Analysis by SEC-MALS-IV
Branching impacts polymer properties such as rheology and stiffness. This note explains the analysis of branching ratio versus molar mass, accomplished by SEC-MALS(-IV).
Troubleshooting LC Separations of Biomolecules, Part 1: Background, and the Meaning of Inertness
How do bioinert and biocompatible liquid chromatography (LC) systems and columns improve separations of biomolecules? How do I know when these systems are required for my separation?
Analysis of Oligonucleotides by SEC
This note demonstrates the ability of a TSKgel® UP-SW2000 SEC column to discriminate oligonucleotides differing by one base in length.
Quantifying CQAs of gene therapy vectors
This note describes how SEC-MALS-UV-RI analysis quantifies multiple critical quality attributes of AAV viral vectors, including concentration/titer, aggregation and empty:full ratio.
Characterization of Hydroxyethyl Starch Using Gel Permeation Chromatography
In this note, the molecular weight averages and molecular weight distributions of two hydroxyethyl starch (HES) samples were successfully demonstrated.