Application Notes: Size-Exclusion Chromatography (SEC)
Characterizing Vaccines with Light Scattering (August 2022)
Light scattering methods inform on essential properties and attributes of vaccines including size, aggregation, stability, interactions, composition, and conformation.
Characterization of Adeno-Associated Viral Vectors
Quality attributes of Adeno-Associated Virus (AAV) vectors are achieved with multi-angle light scattering coupled to separation methods and high-throughput dynamic light scattering.
Protein Aggregate Analysis by SEC
mAb and ADC aggregate analysis by SEC is introduced. Separation was improved by suppressing secondary interactions using high concentrations of corrosive salts.
Liposome Structure and Concentration by FFF-MALS-DLS
Field-flow fractionation with online static and dynamic light scattering constitutes a powerful and versatile system for nano-pharmaceutical characterization.
Aggregate Analysis of Recombinant Human Growth Hormone v2
The SEC separation of recombinant human growth hormone somatotropin with good peak shape and separation of high molecular weight aggregate was observed.
White Paper: Size-Exclusion Chromatography (SEC) for the Determination of Protein Hydrodynamic Radius
Protein standards explored with known hydrodynamic radii, show how this can determine ideal size-exclusion conditions for optimal method performance using Biozen columns.
The Effect of Column Internal Diameter for Robust SEC Methods
This application note explores the effect of column internal diameter (4.8 and 7.6 mm IDs) for robust size-exclusion chromatography methods.
Aggregate Analysis of an IgG2 Monoclonal Antibody Using a Biozen dSEC-2 LC Column
Exploring the use of a standard phosphate containing buffer for aggregate analysis of an IgG2 mAb, Panitumumab, which has known recovery issues due to its hydrophobicity
Improving Resolution for Size-Exclusion Chromatography Methods
Size-exclusion method improvements by running at a lower flow rate for the aggregate analysis of NIST mAb using a Biozen dSEC-2 column
Assessing Column Lifetime of a UHPLC Biozen dSEC-2 LC Column
The robustness of a sub-2 µm Biozen dSEC-2 column is demonstrated by displaying over 300 injections of a protein sample (Myoglobin).