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A Guide to Software-as-a-Service LIMS (June 2024)
June 11th 2024A subscription-based Laboratory Information Management System (LIMS), hosted in the cloud and delivered via the Internet, is a secure, scalable, and cost-effective alternative to traditional software licensing, installation, maintenance, validation, and management. Here’s what you need to know to determine if the software-as-a-service (SaaS) delivery model for a LIMS is right for your organization.
How AI-Powered Analytics Transforms LIMS Data into Valuable, Action-Ready Insights (June 2024)
June 11th 2024Advanced analytics in the lab can extract enormous value from an existing asset – your LIMS data. Discover how a lab-specific, LIMS-ready advanced analytics solution can solve the multi-challenges of data access, visibility, volume, and speed to yield complete data views and endless insights.
Inside the Lab of the Future (June 2024)
June 11th 2024Drive growth by establishing integrated, AI-fueled lab systems and a data-driven corporate culture that’s ready and resilient, no matter what business challenges emerge tomorrow. Evolving to a Lab of the Future takes the right technologies, systems, and expert guidance.
Protein Therapeutics: Antibody Characterization with ECD
June 10th 2024Labile post-translational modifications are better preserved using ECD than conventional MS fragmentation techniques such as collision-induced dissociation (CID). Common modifications, such as glycosylation and phosphorylation, can impact the safety, efficacy, and binding activity of monoclonal antibodies but are challenging to identify. Stephen Sciuto from Agilent Technologies and colleagues analyzed tryptic digests of NIST mAb and Infliximab using an 6545XT Q-TOF mass spectrometer in ExD or CID mode. ECD provided higher sequence coverage than CID, reaching 100% for glycosylated peptides in Infliximab. The ability to pinpoint glycosylation sites using fragment ions demonstrates the power of ECD for characterizing glycopeptides.
Monoclonal Antibodies: Faster Analysis with Microdroplets
June 10th 2024The first reported combination of microdroplet reactions with ECD fragmentation offers a quicker way to analyze monoclonal antibodies for disease treatments. Thomas Walker from Agilent Technologies and co-workers demonstrate the use of an Agilent Jet Stream electrospray ionization source to facilitate in-spray chemical reduction and protease digestion of monoclonal antibodies. Downstream fragmentation of the microdroplet reaction products with ECD provided rapid characterization of intact antibodies in minutes. Efficient ECD fragmentation yielded rich sequence information including evidence of disulfide bond connectivity and confirmation of key sequences called complementary determining regions. These results highlight this method’s potential for fast and cost-effective antibody characterization with minimal sample preparation.
CE-MS Based Sample to Result Workflow for biosimilars (June 2024)
June 7th 2024In this study, we present an ion-pairing-free method for AXPs analysis using microchip CE-MS. This fast, simple method achieves baseline resolution for Adenosine, AMP, ADP, and ATP. Excellent linearity and sensitivity are observed in human plasma.
Analysis of mononucleotides using microchip CE-MS (June 2024)
June 7th 2024In this study, we present an ion-pairing-free method for AXPs analysis using microchip CE-MS. This fast, simple method achieves baseline resolution for Adenosine, AMP, ADP, and ATP. Excellent linearity and sensitivity are observed in human plasma.
Charge Variant Analysis Kit Delivers Superior Performance
June 7th 2024The Charge Variant Analysis (CVA) kit offers superior performance over our first-generation Intact Antibodies kit. With the CVA kit, users can expect greater peak resolution and the ability to properly analyze proteins in their fully native states.