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Practical Implementation of ICH Q14 Guidelines in Digital Method Development
August 9th 2024Poster discussing what the ICH Q14 guidelines entail and how AutoChrom can help implement these principles. The poster includes details about the ICH Q14 guidelines and specific details about how tools and features within AutoChrom can help implement ICH Q14 principles.
More Sensitive Quantification of PFAS by LC/MS with the Agilent 1260 Infinity II Hybrid Multisampler
August 6th 2024This application note demonstrates the use of the Agilent 1260 Infinity II Hybrid Multisampler for the analysis of per- and polyfluorinated alkyl substances (PFAS). The Hybrid Multisampler in feed injection mode was used with optimized Feed Speed for the sample injection to trap and enrich the compounds on the column. This avoided peak broadening and breakthrough of the early-eluting polar PFAS compounds. The less polar PFAS compounds can be enriched for more sensitive quantification.
Oligonucleotides – Drugs for the Future
August 6th 2024Due to their ability to tackle targets that were considered “undruggable”, oligonucleotides represent a key cornerstone of the future of personalized medicine. This infographic presents the different types of therapeutic oligonucleotides and highlights some innovative solutions for their analysis.
Ultra-Trace Quantification of Per- and Polyfluoroalkyl Substances (PFAS) in Drinking Water
August 6th 2024The US EPA has released interim health advisory limits (HALs) of 4 and 20 pg/L in drinking water for the PFAS perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS), respectively. This application note addresses PFAS testing at these challenging new levels in aqueous samples using optimized solid-phase sample extraction and LC/MS/MS analysis with an Agilent 1290 Infinity II liquid chromatography (LC) system coupled to an Agilent 6495C triple quadrupole (TQ) mass spectrometer.
Mass Information on Charge Variants in Minutes, Not Weeks
August 2nd 2024Explore how an integrated, single platform imaged capillary isoelectric focusing (icIEF)-UV/MS workflow with the Intabio ZT system aims to tackle the challenge of charge heterogeneity profiling with a disruptive strategy that allows for separation, quantitation, and identification of individual charge variants to be achieved in minutes on a single platform.
Optimizing Oligonucleotide Analysis
August 1st 2024Due to their ability to tackle targets that were considered undruggable, oligonucleotides represent a key cornerstone of the future of personalized medicine. This infographic presents the different types of therapeutic oligonucleotides and highlights some innovative solutions for their analysis.
Contamination Under Control for Reproducible Amplification
July 30th 2024Sample preparation tools such as pipettes and lab water systems are vital for nucleic acid techniques (NAT), where high quality, contamination-free reagents and precise volume measurements are essential for reproducibility and accuracy.
Preparation of Bioassays & Serial Dilutions in Microplates
July 30th 2024Bioassay techniques are pivotal in quantifying and characterizing the effects of substances on biological systems. Utilized extensively in biotherapeutics, diagnostics, and environmental sciences, these assays range from ELISA for protein and antibody detection to flow cytometry for cell health analysis. Bio-layer interferometry and cell-based assays further elucidate drug-receptor interactions.
Hydrophobic Interaction Chromatography: A Platform for Characterizing Antibody-Drug Conjugates
July 30th 2024Characterizing the ratio of tumor-killing payload to cancer-binding antibody (DAR) is a critical quality attribute that helps determine the potential potency of a given antibody-drug conjugate (ADC) candidate. The TSKgel® HIC-ADC Butyl column, developed by Tosoh Bioscience, enables improved HIC-based separations for quantifying the DAR of ADCs.
Lab essentials for tailored sample preparation
July 30th 2024Sartorius provides an extensive selection of lab essentials tailored for the initial stages of sample handling and preanalytical processes in quality assurance labs, catering to diverse characterization requirements. Our lineup includes accurate lab scales, high-purity water purification systems, superior filtering equipment, and comfortable pipettes and tips
Analyze Charge Variants in Antibody-Based Therapeutics Using Cation Exchange Chromatography
July 18th 2024This application note discusses the latest development on high performance cation exchange bio-separation columns for determining charge variants in protein-based biotherapeutics, as well as the strategy on method development.
The Power of HPLC/SFC Control and AQbD in One Software
July 17th 2024Download this infographic for a quick overview of Shimadzu’s LabSolutions Method Development (MD) software, an effective and automated software solution for U/HPLC method development that uses analytical quality by design (AQbD) principles in its workflow.