Application Notes

The Focus LC high-performance liquid chromatography (HPLC) system is small enough to be easily placed near the sample source and powerful enough to be used in any state-of-the-art laboratory. It’s optimized for low solvent consumption, waste reduction, and energy conservation, and delivers the performance and reliability you need for the highest confidence in your daily results.

InFocus enables real-time in-line sampling, ensuring process integrity throughout the workflow, including inside a hood or next to a reaction vessel. Scientific breakthroughs and process optimizations require fast, accurate, and reliable data. As a Process Analytical Technology (PAT) tool, the InFocus enables real-time, in-line, on-line, and at-line analysis, allowing scientists and engineers to detect inefficiencies, optimize reactions, and improve product quality without delays.

AutoFocus autosampler allows automated and unattended introduction of multiple standards and samples to the Axcend Focus LC for separation and analysis. The automated sampling system streamlines precision sample injection, improving efficiency and throughput. Designed for effortless operation and maintenance, AutoFocus offers excellent performance, making it the ideal autosampler for unattended analyses.

A full-stack capillary HPLC system, such as the one recently launched by Axcend, integrates several innovative features that set it apart from traditional HPLC systems. This compact system includes a 40-vial/96-well plate autosampler, an in-line process analytical technology (PAT) monitoring system, and a full-spectrum diode array detector (DAD). These components work together to enable real-time data analysis at the point of sampling, significantly enhancing productivity and precision. One of the most remarkable aspects of this system is its small footprint, which optimizes valuable lab and production space while reducing costs and environmental impact (the base measures 13" X 9"). The low-flow design minimizes solvent consumption and hazardous waste, aligning with sustainable laboratory practices. Additionally, the system's ability to perform HPLC anywhere—whether inside a hood or next to a reaction vessel—provides unparalleled flexibility for scientists.

Pittcon took place from March 1 to 5, 2025, in Boston, Massachusetts. This premier event brought together professionals from various laboratory science disciplines to share research findings, discuss advancements, and collaborate on best practices. In this video interview, we speak with Katelynn Perrault Uptmor, Xiao Su, Chris Palmer, and Long Luo, all of whom attended Pittcon this year and delivered important talks that provide insight into the state of separation science.

As a leader responsible for metabolite identification in your organization, what keeps you awake at night? SCIEX can give you peace of mind by helping you . . .

Here we discuss a software-assisted drug metabolite identification and characterization workflow. The approach allows scientists to have increased confidence in the process.

The dream state If we lived in an ideal world, it would be possible to unambiguously identify metabolites using a single analytical experiment. This analytical technique would need to be efficient and easily generate the information needed from a routine assay that is also robust, enabling confident decision-making during drug discovery. At SCIEX, we believe that metabolite identification using the ZenoTOF 7600 system gets close to this dream state.

This application note presents the development and validation of a multi-component method for the analysis of 21 PFAS compounds in chicken eggs.

This application note presents the development and validation of a multiresidue method for the analysis of PFAS in baby food.

This application note presents the development and validation of a multiresidue method for the analysis of PFAS residues in beef, tuna, and shrimp.

This application note presents the development and validation of a multiresidue method for the analysis of PFAS in fruits, vegetables, and juices.

GRPC peptides are found in the entire body. Isolation of seven classic GRPC peptides initiating in the GI tract could implicate disease.

The diterpenoid, paclitaxel, which was identified as a potent chemotherapy agent for breast and ovarian cancer originates from the Pacific Yew tree. The isolation of paclitaxel from its major impurities is shown with the use of Hamilton’s PRP-1 (5 µm) HPLC column.

The identification benzodiazepine metabolites can be highly valuable in detecting abuse. Metabolites of oxazepam are found containing both hydroxyl and glucuronides.

Plasticizers added to water bottles to add stability to the bottles can be unknowingly extracted in drinks leading to possible endocrine interactions.

The fast identification of tocopherols are important for various applications including free radical scavenging for human health and food stuff preservation.

Isolation of capsaicin for medicinal uses is easily achieved with the PRP-C18 RP-HPLC resin.

Discover how the HC-75 series uses a ligand exchange mechanism in order to interact more or less strongly with the analytes of interest.

Lee Ferguson, a Professor of Civil and Environmental Engineering at Duke University, and his group are developing and applying analytical methods to measure organic pollutants in the environment, with a focus on high-resolution mass spectrometry (HRMS) for detecting and quantifying emerging contaminants in wastewater and drinking water. In this video interview, we speak with Ferguson about his laboratory’s work accurately tracing these contaminants in complex environmental matrices.

This brief communitation hightlights the assessment of Hamilton PRP-3 HPLC column attributes when analyzing biologically active peptides.

Per- and polyfluoroalkyl substances demand precise detection. YMC-Triart C18 columns ensure superior separation and accurate quantification, even in complex matrices.

This application note presents an automated method for generating calibration curves using an autosampler’s dilution function. It offers practical examples demonstrating a simple setup, enhanced productivity, accuracy and reliability, with a focus on eliminating manual dilution for greater efficiency in HPLC analyses.

Part 2 delves into advanced applications using organic solvents with the HPLC autosampler dilution function. It details procedures for mixed standard solutions, highlighting automation’s role in reducing errors, saving labor and promoting efficient laboratory practices.

In BioTech R&D, overcoming data fragmentation is crucial as it hinders innovation, leads to redundant efforts, and wastes resources.

When high-quality, cost-effective impurity reference standards are unavailable, RRFs provide a practical alternative for impurity quantification.