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The first small footprint full-stack HPLC System
March 21st 2025A full-stack capillary HPLC system, such as the one recently launched by Axcend, integrates several innovative features that set it apart from traditional HPLC systems. This compact system includes a 40-vial/96-well plate autosampler, an in-line process analytical technology (PAT) monitoring system, and a full-spectrum diode array detector (DAD). These components work together to enable real-time data analysis at the point of sampling, significantly enhancing productivity and precision. One of the most remarkable aspects of this system is its small footprint, which optimizes valuable lab and production space while reducing costs and environmental impact (the base measures 13" X 9"). The low-flow design minimizes solvent consumption and hazardous waste, aligning with sustainable laboratory practices. Additionally, the system's ability to perform HPLC anywhere—whether inside a hood or next to a reaction vessel—provides unparalleled flexibility for scientists.
Important Talks You May Have Missed at Pittcon 2025
March 20th 2025Pittcon took place from March 1 to 5, 2025, in Boston, Massachusetts. This premier event brought together professionals from various laboratory science disciplines to share research findings, discuss advancements, and collaborate on best practices. In this video interview, we speak with Katelynn Perrault Uptmor, Xiao Su, Chris Palmer, and Long Luo, all of whom attended Pittcon this year and delivered important talks that provide insight into the state of separation science.
Is “right first time, every time” a pipedream for metabolite identification by LC-MS?
March 18th 2025The dream state If we lived in an ideal world, it would be possible to unambiguously identify metabolites using a single analytical experiment. This analytical technique would need to be efficient and easily generate the information needed from a routine assay that is also robust, enabling confident decision-making during drug discovery. At SCIEX, we believe that metabolite identification using the ZenoTOF 7600 system gets close to this dream state.
Pick Your Poison. Isolation of Paclitaxel (Mar 2025)
March 7th 2025The diterpenoid, paclitaxel, which was identified as a potent chemotherapy agent for breast and ovarian cancer originates from the Pacific Yew tree. The isolation of paclitaxel from its major impurities is shown with the use of Hamilton’s PRP-1 (5 µm) HPLC column.
The Role of Mass Spectrometry and Chromatography in Mitigating PFAS in the Environment
March 7th 2025Lee Ferguson, a Professor of Civil and Environmental Engineering at Duke University, and his group are developing and applying analytical methods to measure organic pollutants in the environment, with a focus on high-resolution mass spectrometry (HRMS) for detecting and quantifying emerging contaminants in wastewater and drinking water. In this video interview, we speak with Ferguson about his laboratory’s work accurately tracing these contaminants in complex environmental matrices.
Efficient Calibration Curve Generation with Automated Dilution: Part 1
March 4th 2025This application note presents an automated method for generating calibration curves using an autosampler’s dilution function. It offers practical examples demonstrating a simple setup, enhanced productivity, accuracy and reliability, with a focus on eliminating manual dilution for greater efficiency in HPLC analyses.
Efficient Calibration Curve Generation with Automated Dilution: Part 2
March 4th 2025Part 2 delves into advanced applications using organic solvents with the HPLC autosampler dilution function. It details procedures for mixed standard solutions, highlighting automation’s role in reducing errors, saving labor and promoting efficient laboratory practices.