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© 2021 MJH Life Sciences and Chromatography Online. All rights reserved.
© 2021 MJH Life Sciences™ and Chromatography Online. All rights reserved.
May 04, 2021
We demonstrate how to achieve deep proteome coverage on HeLa cell lysate using a high pH reversed-phase fractionation strategy in combination with nano flow LC–MS.
The bioZen high pH fractionation HPLC column provides superior performance using titanium hardware, minimizing nonspecific adsorption for peptide identifications.
In this technical note we explore column miniaturization effects attained by reducing column ID from 300 μm to 75 μm and its effects on sensitivity.
In this application we show reproducible results across multiple columns as well as the robust ability of the bioZen 2.6 μm XB-C18 column after 100 injections.
April 30, 2021
Charge variants of 28 mAbs were analyzed under MS-compatible conditions using YMC’s BioPro IEX SF column. Each mAb of different pI was separated from its variants
In reference to the Japanese Pharmacopeia, YMC-Triart Bio C18 was used to analyze the peptide leuprorelin (GnRH analogue) and separate it from its degradation products.
In this application note, YMC’s dedicated SEC column for antibodies, YMC-SEC MAB, has been used to develop a method for bevacizumab using MALLS detection.
Mipomersen was analyzed by LC–HRMS using a biocompatible YMC-Triart C8 metal-free column ensuring symmetric peaks, high sensitivity, and no carryover effects.