Axcend

Adapting high-performance liquid chromatography (HPLC) methods to the capillary scale opens new possibilities for pharmaceutical analysis and sustainability. This paper, featuring a collaboration with the University of Pavia and Axcend, examines real-world method conversions that preserve performance while minimizing waste. Case studies include detection of a polar impurity in pediatric ibuprofen mini-tablets using a micro-HPLC-UV method, and a USP <621> guided adaptation for hydrochlorothiazide demonstrating over 99% solvent reduction. Practical guidelines, expected trade-offs, and data from both academic and regulated environments will offer a roadmap to adapting validated pharmaceutical monographs to the capillary scale without the need for revalidation.

Webinar Date/Time: Mon, Nov 3, 2025 11:00 AM EST

As pharmaceutical laboratories face increasing pressure to improve efficiency, sustainability, and analytical precision, the demand for highly repeatable, resource-efficient instrumentation is on the rise. Capillary-scale liquid chromatography (LC) is reshaping expectations, enabling labs to achieve high-impact results without added overhead.

Webinar Date/Time: Mon, Jun 9, 2025 11:00 AM EDT

The Axcend FocusArray is the ideal choice for your challenging liquid chromatography requirements. The only diode array detectr for capillary HPLC offers a full three orders of magnitude linearity needed to identify trace elements in the presence of your target analytes.

The Focus LC high-performance liquid chromatography (HPLC) system is small enough to be easily placed near the sample source and powerful enough to be used in any state-of-the-art laboratory. It’s optimized for low solvent consumption, waste reduction, and energy conservation, and delivers the performance and reliability you need for the highest confidence in your daily results.

InFocus enables real-time in-line sampling, ensuring process integrity throughout the workflow, including inside a hood or next to a reaction vessel. Scientific breakthroughs and process optimizations require fast, accurate, and reliable data. As a Process Analytical Technology (PAT) tool, the InFocus enables real-time, in-line, on-line, and at-line analysis, allowing scientists and engineers to detect inefficiencies, optimize reactions, and improve product quality without delays.

AutoFocus autosampler allows automated and unattended introduction of multiple standards and samples to the Axcend Focus LC for separation and analysis. The automated sampling system streamlines precision sample injection, improving efficiency and throughput. Designed for effortless operation and maintenance, AutoFocus offers excellent performance, making it the ideal autosampler for unattended analyses.

A full-stack capillary HPLC system, such as the one recently launched by Axcend, integrates several innovative features that set it apart from traditional HPLC systems. This compact system includes a 40-vial/96-well plate autosampler, an in-line process analytical technology (PAT) monitoring system, and a full-spectrum diode array detector (DAD). These components work together to enable real-time data analysis at the point of sampling, significantly enhancing productivity and precision. One of the most remarkable aspects of this system is its small footprint, which optimizes valuable lab and production space while reducing costs and environmental impact (the base measures 13" X 9"). The low-flow design minimizes solvent consumption and hazardous waste, aligning with sustainable laboratory practices. Additionally, the system's ability to perform HPLC anywhere—whether inside a hood or next to a reaction vessel—provides unparalleled flexibility for scientists.

Webinar Date/Time: Wed, Mar 19, 2025 11:00 AM EDT

This application note demonstrates the use of a compact portable capillary liquid chromatograph, the Axcend Focus LC, coupled to an Agilent Ultivo triple quadrupole mass spectrometer for quantitative analysis of pharmaceutical drugs in model aqueous samples.

Now there's an single-purpose HPLC with a simplified UI that makes it possible for anyone to perform HPLC testing with a sinple one-button pass/fail test function.

Welcome to our comprehensive selection of High-Performance Liquid Chromatography (HPLC) cartridges. Designed for precision and reliability, our cartridges cater to diverse analytical needs, ensuring optimal performance in your laboratory processes.

The solution is an automated reaction monitoring system capable of unattended sampling, identification, and quantification of reaction components via HPLC. Axcend system is the only separations Process Analytical Technology (PAT) system that’s small enough to be placed in a hood or immediately adjacent to the reaction vessel.

Webinar Date/Time: Fri, Nov 8, 2024 11:00 AM EST

The Axcend Focus LC®, a portable capillary liquid chromatograph, coupled to an Agilent Ultivo triple quadrupole mass spectrometer for analysis of pharmaceutical drugs.

Compact HPLC Specification

Axcend’s revolutionary line-up of eco-friendly, compact and portable HPLC instruments and accessories are engineered to deliver consistent, high-quality performance, ensuring precise and dependable analytical results. Compact and portable, the Axcend Focus LC deliver HPLC Anywhere™.

A demonstration of emptying a waste container of a compact and portable LC system.

The Axcend Focus LC® stands out as an eco-friendly high-performance liquid chromatograph (HPLC) due to its innovative design and sustainable features. This compact system uses significantly less solvent, consuming under 10 mL per week, and produces roughly the same amount of waste. Additionally, its energy consumption is a fraction of that of larger, bench-top LCs. By minimizing solvent use and waste production, the Axcend Focus LC not only reduces operational costs but also aligns with green analytical chemistry principles, making it an environmentally responsible choice for laboratories

The current trend in laboratory equipment design is the miniaturization of laboratory instruments. Smaller-scale HPLC instruments offer benefits that cannot be matched by analytical-scale equipment, especially in the areas of portability, reduced fluid volumes, and reduced operating costs. Yet, the miniaturization of laboratory equipment has brought with it a unique set of challenges, including transferring methods to compact LC. Capillary LC expands the use of LC to applications not currently done using conventional LC in a wide array of application areas, including pharmaceutical, food and beverage, petrochemical, environmental, and oil and gas. Greg Ward, Axcend’s CEO wrote, “Customers want an HPLC system with a small footprint, low flow rates and green chemistry.” Join his podcast where he shares method transfer in these application areas.

The current trend in laboratory equipment design is the miniaturization of laboratory instruments. Smaller-scale HPLC instruments offer benefits that cannot be matched by analytical-scale equipment, especially in the areas of portability, reduced fluid volumes, and reduced operating costs. Yet, the miniaturization of laboratory equipment has brought with it a unique set of challenges, including transferring methods to compact LC. Capillary LC expands the use of LC to applications not currently done using conventional LC in a wide array of application areas, including pharmaceutical, food and beverage, petrochemical, environmental, and oil and gas. Greg Ward, Axcend’s CEO wrote, “Customers want an HPLC system with a small footprint, low flow rates and green chemistry.” Join his podcast where he shares method transfer in these application areas.

The Focus LC® separated PAHs in ~12 and ~15 min, with separate methods on short capillary columns packed with 1.7 µm monomeric and 3.0 diameter polymeric C18 particles.

The separation of five test proteins in the range of 5.8 to 45 kDa is shown using reversed phase (RP) conditions.

Real time determination of the presence of MDA in an aviation turbine fuel sample at installation level or in a field environment outside of a laboratory.

A point-of-care LC system that is compact, reliable, and easy to use. The Axcend Focus LC is efficient, allowing for informed decisions at the time of need.

Repeatable analyses of API dissolution. An Extra Strength Excedrin® analgesic tablet was dissolved in a 200 mL solution (97% water; 3% ACN; and <0.1% TFA).

Portable HPLC to detect and separate 5 cannabinoids present in 50 mg of a hemp-variant of cannabis flowers and 12 additional cannabinoids present in a Restek standard.

*Tuesday, June 1, 2021 at 12:30pm BST| 1:30pm CEST| 7:30am EDT & Wednesday, June 2, 2021 at 12:30pm BST| 1:30pm CEST| 7:30am EDT* John Knox played a pivotal role in the evolution and understanding of both gas and liquid chromatography and is perhaps best known for the development of the Knox Equation, which describes the different contributions to overall band broadening of a solute. *On Demand June 2, 2021*