
LCGC North America
Delivering samples to the analytical column in ‘clean’ mobile phase is important for robust methods and high quality results.

Dwight R. Stoll is the editor of “LC Troubleshooting”. Stoll is a professor and the co-chair of chemistry at Gustavus Adolphus College in St. Peter, Minnesota, USA. His primary research focus is on the development of 2D-LC for both targeted and untargeted analyses. He has authored or coauthored more than 75 peer-reviewed publications and four book chapters in separation science and more than 100 conference presentations. He is also a member of LCGC’s editorial advisory board. Direct correspondence to: [email protected]

LCGC North America
Delivering samples to the analytical column in ‘clean’ mobile phase is important for robust methods and high quality results.

The Application Notebook
In his article, published in 2000, David V. McCalley found large increases in the efficiency for basic compounds at elevated temperature

LCGC North America
Despite the utility of high performance liquid chromatography (HPLC) simulators, we found that all the free and low-cost simulators were outdated or had extremely limited functionality, so we created one that addressed these shortcomings. We developed a sophisticated, open-source HPLC simulator that is available for free as well as a version for Android users called "HPLC Simulator." Here we discuss a few questions that the simulator can help answer: Why are peaks narrower in gradient elution? What is gradient delay and how does it affect a separation?

The Application Notebook
ZirChrom Separations, Inc.

LCGC North America
The guest authors describe an optimization procedure to achieve the highest plate count in a given analysis time.