Sam Whitmarsh

The panel cover protecting peptide recovery, from mobile-phase pH and diluent buffers to inert column hardware.

Piotr Alvarez explains how his lab uses 2D-LC to enable MS compatibility with legacy methods and to confirm peak purity through orthogonal separation.

How can analysts resolve impurities that differ by a single mass unit? Experts discuss deamidation, racemization, HRMS, and 2D-LC for GLP-1 peptides.

The panel walks through confirming peptide identity–from HRMS and LC–MS/MS sequencing to amino acid analysis and D/L-isomer verification.

Nitish Sharma and Piotr Alvarez compare reversed-phase separations of small molecules vs. peptides, from pI value and hydrophobicity to impurity profiles.

Meet the expert panel exploring the challenges involved in GLP-1 analysis in this LCGC International Peer Exchange®.