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SEP21 PreviewThe 14th Congress of the French-speaking Association for Separative Sciences (SEP21) will take place at the Porte de Versailles in Paris, France, from 5–7 October 2021. This preview explores what visitors can look forward to from the conference.
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Separation of All Classes of Carbohydrates by HPAEC-PADHigh performance anion-exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) is a potential method of choice for the analysis of carbohydrates.
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Simultaneous Determination of 17 Residual Monomers in Adhesives by Gas Chromatography–Mass SpectrometryAdhesives often contain toxic materials like residual monomers; here, we demonstrate quantitative analysis of residual monomers in adhesives using GC–MS.
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New Advancements in Biopharma Protein Characterization Using HRIMWebinar Date/Time: Friday, September 29, 2023 at 11am EDT | 8am PDT | 4pm BST | 5pm CEST
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Nexera SFC Systems Combine Analysis, Purification & ExtractionAre you working with LC and GC in pharmaceutical discovery, development, or QC/QA? Looking for higher speeds, increased sensitivity, reduced solvent consumption and automated sample prep/collection?
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The LCGC Blog: Whence the Next Generation of Macromolecular Separations Scientists?It is of paramount importance that companies develop their in-house macromolecular separations expertise, not only polymer manufacturing companies but also those employing macromolecules for any of a host of applications, from drug-delivery and tablet coatings to ink-jet printer formulations and food additives, to name but a few.
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Comparing the Chemical Profiles of Plant-Based and Traditional Meats Using GC–MS-Based MetabolomicsUntargeted GC–MS metabolomics with sample derivatization is shown here to be effective for measuring the chemical profiles of traditional and plant-based meat products.
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High-Throughput Profiling of Long Chain Fatty Acids and Oxylipins by LC–MSLong chain fatty acids (LCFAs) function as a source of metabolic energy, substrates for membrane biogenesis, and storage of metabolic energy. Oxylipins, oxygenated derivatives of LCFAs, regulate the activity of many cellular processes. Existing methods for the analysis of LCFAs and oxylipins have limited compound coverage and sensitivity that, therefore, prevent their application in biological studies. In this work, we developed a high-throughput LC–MS method for analysis of 51 LCFAs and oxylipins. LCFAs and oxylipins were first extracted from biological samples via solid-phase extraction. The extracted molecules were analyzed by targeted comparative metabolomics. Saturated and monounsaturated LCFAs were analyzed in single ion reaction mode, while polyunsaturated LCFAs and oxylipins were analyzed in multiple reaction monitoring mode. Using this method, we successfully quantified 31 LCFAs and oxylipins from mouse livers.
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Mass Spectrometry–Based Process Analytical Technologies for Cell TherapiesEven compared to other biopharmaceuticals, chimeric antigen receptor (CAR) T-cell therapies are particularly complex. Mass spectrometry (MS)–based process analytical technologies can be a powerful tool in identifying and monitoring key attributes throughout the manufacturing process.
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Lowering Limits of Quantitation in LC–MS/MS Analyses for Food Safety and Environmental ApplicationsHow to lower the limits of quantitation as the regulatory landscape continues to evolve.
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Liquid Chromatography–Mass Spectrometry Analysis of Hop-Derived Humulone and Isohumulone Constituents in Beer: The Bitter Truth of Hops Utilization During BrewingA rapid LC–MS method using ESI coupled with SIM-MS for analysis of humulone and isohumulone content in beer is described.
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Diving into the Structural Details of In Vitro Transcribed mRNA Using Liquid Chromatography–Mass Spectrometry-Based Oligonucleotide ProfilingAn overview of mRNA-based vaccine production and a discussion on the methods that are currently in use to check the quality of these vaccines.
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Probe Electrospray Ionization-Mass Spectrometry and Isotope Dilution Approaches for Ultrafast Measurement of MetforminIn this study, a probe electrospray ionization coupled to mass spectrometry (PESI-MS) method for ultra-rapid determination of MtF in plasma by isotope dilution was developed.
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Chromatographic Innovations for Cell TherapiesThe complexity and challenges of developing, manufacturing, and controlling cell therapies offer today’s chromatographic scientists new opportunities to join the journey of discovery and innovation needed to develop and commercialize this new drug modality. This article explores the latest developments and highlights the importance of new capillary electrophoresis–mass spectrometry (CE–MS)-based approaches to understand the efficiency of cell therapy production.
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Event Preview: Recent Advances in Gas ChromatographyRecent Advances in Gas Chromatography Event Preview and other important events for chromatographers
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Current Good Manufacturing Practice (cGMP): An Overview for Analytical ChemistsThis article provides a high-level overview of Current Good Manufacturing Practice (cGMP) regulations and related public quality standards.
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Nontargeted Screening as an Essential Tool for Drinking Water Quality MonitoringLiquid chromatography coupled with high-resolution mass spectrometry (LC–HRMS) is used in combination with a comprehensive data analysis workflow to screen water samples for potentially hazardous transformation products from organic micropollutants to determine the efficacy of different water treatment methods.
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Another 10 Ways to Spoil a CE SeparationThis month's "CE Currents" continues an examination of problems that users may find in routine CE work. Readers can avoid these problems by adopting the working practices suggested by the authors.
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Novel LC–MS/MS Method with a Dual ESI and APCI Ion Source for Analysis of California-Regulated Pesticides and Mycotoxins in Medium-Chain Triglyceride (MCT) Oil Cannabis TincturesA novel liquid chromatography–tandem mass spectrometry (LC–MS/MS) method with a dual electrospray ionization (ESI) and atmospheric-pressure chemical ionization (APCI) source was developed for analysis of 66 pesticides and 5 mycotoxins regulated by the State of California in medium-chain triglyceride (MCT) oil cannabis tinctures. The limits of quantitation (LOQs) of all of the pesticides and mycotoxins were well below California state action limits of these analytes in cannabis-based non-inhalable or edible products. A total of 62 (out of 66) pesticides and 5 mycotoxins were analyzed using LC–MS/MS with an ESI source, and the remaining 4 pesticides were determined using LC–MS/MS with an APCI source. A simple, fast, and cheap acetonitrile solvent extraction method was used for sample preparation to get good recovery and achieve high throughput for this analysis. For late-eluted analytes, a number of internal standards were used to compensate for ion suppression effects from the hydrophobic matrix.
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Long-Term Analyses with Capillary ElectrophoresisThis month's installment of "CE Currents" deals with problems that may arise when using capillary electrophoresis for unattended, long-term operation, such as sample carryover, evaporation effects, capillary conditioning, capillary surface changes, buffer handling, capillary breakage, and detector lamp deterioration.
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Possibilities and Limitations of Aqueous Temperature Responsive Liquid Chromatography in Comprehensive 2D–LCAnalyzing representative standard mixtures, APIs, and synthetic impurities shows that when TRLC is combined with RPLC in 2D-LC, separation performance is improved. We explain why.
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LCGC Blog: Forensics Laboratories Underassess Uncertainty in Blood Alcohol DeterminationsThe level of uncertainty provided by most forensic laboratories for reported blood alcohol results has been woefully underassessed. Not only is this bad science, but someone’s civil liberties may be at stake.
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Novel LC–MS/MS Method with a Dual ESI and APCI Ion Source for Analysis of California-Regulated Pesticides and Mycotoxins in Medium-Chain Triglyceride (MCT) Oil Cannabis TincturesA novel liquid chromatography–tandem mass spectrometry (LC–MS/MS) method with a dual electrospray ionization (ESI) and atmospheric-pressure chemical ionization (APCI) source was developed for analysis of 66 pesticides and 5 mycotoxins regulated by the State of California in medium-chain triglyceride (MCT) oil cannabis tinctures. The limits of quantitation (LOQs) of all of the pesticides and mycotoxins were well below California state action limits of these analytes in cannabis-based non-inhalable or edible products. A total of 62 (out of 66) pesticides and 5 mycotoxins were analyzed using LC–MS/MS with an ESI source, and the remaining 4 pesticides were determined using LC–MS/MS with an APCI source. A simple, fast, and cheap acetonitrile solvent extraction method was used for sample preparation to get good recovery and achieve high throughput for this analysis. For late-eluted analytes, a number of internal standards were used to compensate for ion suppression effects from the hydrophobic matrix.
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Optimization of Determination Method of Cooling Agents in Cigarette Tipping Paper by Gas ChromatographyThis GC–FID method enables accurate determination of cooling agents, which are flavor compounds commonly used in cigarette tipping paper.
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In Memory of Pavel Jandera, Leading ChromatographerProfessor Pavel Jandera passed away suddenly and unexpectedly on August 5, 2021. He was one of the most famous Czech analytical chemists. He was 77 years old.
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Application of Liquid- and Supercritical Fluid Chromatography Coupled with High-Resolution Mass Spectrometry for the Analysis of Short-, Medium-, and Long-Chain Chlorinated Paraffins in Dietary SupplementsScientists used supercritical fluid chromatography (SFC) and ultrahigh-pressure liquid chromatography (UHPLC)—both coupled with high-resolution mass spectrometry (HRMS)—to analyze various types of chlorinated paraffins (CPs) in fish oil-based dietary supplements.
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Determination of Organophosphate Esters in Water Samples Using Gas Chromatography–Mass Spectrometry (GC–MS) and Magnetic Solid-Phase Extraction (SPE) Based on Multi-Walled Carbon Nanotubes (MWCNTs)A GC–MS method, coupled with magnetic SPE with multi-walled carbon-nanotube (MWCNT)-coated iron oxide as the adsorbent, was developed for analyzing organophosphate esters in environmental water samples.
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Advances in Gas Chromatography for Optimal Dioxin GC SeparationHow can GC overcome the complexity of dioxin separation and analysis?
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Bioanalytical Analysis of Small-Molecule Drugs and Metabolites in Physiological Samples by LC–MS, Part 2: Sample PreparationThis concise yet comprehensive overview of sample preparation for bioanalysis looks at sample preparation fundamentals, best practices, and modern trends—all illustrated with a case study.
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Analysis of Proteins, Biologics, and Nanoparticles in Biological Fluids Using Asymmetrical Flow Field-Flow FractionationAspects of applying AF4 to plasma, serum, milk, and cerebrospinal fluid in the field of analysis and characterization of proteins, biologics, and nanoparticles in biological fluids are reviewed.
