Author
Latest:
Portable and Field‑Deployable Liquid Chromatography for Environmental StudiesLiquid chromatography (LC) is a platform technology amenable to portable and “at-site” or deployable applications. This has awoken end-users to new possibilities and potential cost savings and process improvements.
Latest:
Rapid Method to Characterize Adeno-Associated Virus (AAV) Therapies Using SEC–FLDA fast and sensitive SEC technique incorporating a wide pore stationary phase with fluorescence detection, and its benefits, are described.
Latest:
An Ultrahigh-Performance Liquid Chromatography Alternative to Animal Testing for Skin SensitizationOECD-compliant amino acid derivative assay (ADRA) testing and method transfer to UHPLC conditions is presented.
Latest:
Method Development Considerations for Intact Monoclonal Antibody Analysis Using UHPLC InstrumentationThe effect of the mobile phase composition, organic alcohols, ion-pair reagents, and different bonding chemistries on mAb separations is discussed.
Latest:
Recent Advances and Applications of Passive Sampling DevicesPassive samplers come in many forms and can be used in various fields. Here, we review multiple advances made to this sampling strategy.
Latest:
Determination of Microplastic Mass Content by Thermal Extraction Desorption Gas Chromatography–Mass SpectrometryA novel analysis technique has been developed for the determination of microplastics (MPs) in complex environmental samples using thermal extraction desorption gas chromatography–mass spectrometry (TED-GC–MS).
Latest:
Analysis of 12 Regulated Mycotoxins in 8 Food Matrices by Single Immunoaffinity Cleanup and Isotope Dilution LC–MS/MSMycotoxins are toxic metabolites produced by fungal species often found in agricultural products. An accurate method for analyzing 12 regulated mycotoxins is described using UHPLC–MS/MS. The method demonstrated limits of quantitation (LOQs) for all analytes below stringent regulatory limits, making the method suitable for routine mycotoxin analysis.
Latest:
The Role of Liquid Chromatography–Tandem Mass Spectrometry-Based Metabolomics in Disease BiologyExamining the role that LC–MS/MS plays in metabolomics, and metabolomics’ growing importance in the field of disease biology.
Latest:
Analysis of Boronic Compounds as Potential Mutagenic Impurities in Drug Substances By GC–MSThis novel gas chromatography (GC)–MS approach enables analysis of the suspected mutagens bis(pinacolato)diboron (BPD) and tetrahydroxydiboron (BBA) found at low-levels in certain intermediates and active pharmaceutical ingredients (APIs), even in the presence of boron from other sources.
Latest:
Exploring Biopharmaceutical Analysis with Compact Capillary Liquid Chromatography InstrumentationA recent trend in the design of LC instrumentation is the move towards miniaturized and portable systems.
Latest:
Physicochemical Methods for Vectors and Ancillary Materials in Cellular and Gene TherapiesCellular and gene therapies (CGTs) have contributed significantly to the improvement of clinical outcomes for patients in the recent years. This paper discusses a range of physicochemical methods that play an important role in the difficult characterization of viral vectors, to meet the unique needs of CGT manufacturing process development, process and product characterization, and the quality control testing of these materials.
Latest:
Evaluation of Kinetic Performance of Reversed-Phase Columns for Protein Separations by Gradient Kinetic PlotsThis article compares the performance of wide-pore silica monolithic, sub-2µm FPP, and SPP columns, addresses the question of whether 1000 Å or 400 Å SPP columns are more suitable for reversed-phase LC-type protein separations, and presents a kinetic performance comparison of different columns.
Latest:
There is No Time to Waste: Low-Pressure Gas Chromatography– Mass Spectrometry is a Proven Solution for Fast, Sensitive, and Robust GC–MS AnalysisLow-pressure gas chromatography (LPGC)–MS should be the first option in many GC–MS applications to provide fast, sensitive, and robust analyses; and the installation, instrumentation, and implementation of LPGC is possible in any GC–MS system.
Latest:
Onsite Environmental Extraction Based on Portable and Affordable Stirred DevicesThis article outlines the relevance of extraction techniques, including exhaustive and non-exhaustive ones, in onsite strategies.
Latest:
Meeting Regulatory Needs in the Characterization of Lipid Nanoparticles for RNA Delivery via FFF-MALSA validated FFF-MALS method aligned with technical specification ISO/TS 21362 for the analysis of lipid-based nanoparticles (LNPs) encapsulating siRNA and mRNA is presented.
Latest:
Enhancing Flame Ionization Detector Capabilities with Post-Column ReactionThe incorporation of a post-column reaction using a 3D-printed, two-stage microreactor is showing groundbreaking performance improvements for flame ionization detection in many gas chromatography applications—and delivers carbon universal response.
Latest:
Gaining New Insights in Advanced Polymeric Materials Using Comprehensive Two-Dimensional Liquid ChromatographyTwo-dimensional LC, with advanced modulation techniques, can advance the analysis of advanced polymeric materials, assisting in characterizing copolymer composition heterogeneity and identifying ingredients in complex products.
Latest:
EAF4-MALS Assessment of Liposomal DoxorubicinWe present electrical asymmetrical flow field-flow fractionation with multi-angle light scattering (EAF4-MALS) to derive parameters of a liposomal form of doxorubicin.
Latest:
Turning Metabolomics Data Processing from a “Black Box” to a “White Box”Extracting thousands of metabolomics features from LC–MS–based metabolomics data is not easy. A new suite of free bioinformatics tools helps address these metabolic peak-picking challenges and thus obtain more accurate biological information.
Latest:
Essential Interview and Job Preparation Tips for Analytical Chemistry Graduate StudentsFresh insights on how to succeed in the interview process to secure your dream job.
Latest:
Untargeted LC–MS Lipidomics with Data Independent Acquisition using Sequential Window Acquisition of All Theoretical Fragment-Ion SpectraA focus on untargeted UHPLC–MS/MS lipidomics with data independent acquisition using SWATH technology. This article explores the wide-ranging potential and peculiarities of SWATH–MS hyphenated with UHPLC for the profiling of lipidomes of various biological samples.
Latest:
Technical Note: Modifying a Standard HPLC Autosampler for On-Line Process MonitoringA simple modification of the standard HPLC autosampler flow path enables automated sampling and analysis of samples outside the autosampler unit when users cannot place the sample container inside because of size restrictions.
Latest:
A Quick and Accurate High Performance Liquid Chromatography (HPLC) Method to Determine the Amount of Trimethylamine in Fish Oil Softgels and Multivitamin Softgels Containing Fish OilA HPLC method was developed to measure trimethylamine in fish oil supplements, to determine if the level of this compound affects product quality attributes such as rancidity.
Latest:
A Hydrophilic Strong Anion-Exchange Hybrid Monolith for Capillary Liquid ChromatographyThis article presents a new hydrophilic SAX hybrid monolithic column prepared by in‑capillary coating 5 μm bare silica particles with functional organic polymers. After preparation conditions were investigated and selected, the resulting column was characterized in detail.
Latest:
A Flash Qualitative Identification Method for the Specific Component in a Mixture Based on Diode Array DetectorThis paper proposes a new method of flash qualitative identification (FQI) to qualitatively identify a certain target component from a mixture within half a second by disusing the analytical column, which is a time-consuming unit in current chromatography instruments. First, a Noised Spectrum Identification (NSI) model was constructed for the data set generated directly by diode array detector (DAD) without the process in an analytical column. Then, a method called vector error algorithm (VEA) was proposed to generate an error according to the DAD data set for a mixture and a specific spectrum for the target component to be identified. A criterion based on the error generated by the VEA is used to give a judgement of whether the specific spectrum exists in the DAD data set. Several simulations demonstrate the high performance of the FQI method, and an experiment for three known materials was carried out to validate the effectiveness of this method. The results show that the NSI model concurs with the real experiment result; therefore, the error generated by the VEA was an effective criterion to identify a specific component qualitatively, and the FQI method could finish the identification task within half a second.
Latest:
Acid-Catalyzed Isomerization of Carbonyls-2,4- dinitrophenylhydrazone in Mainstream Smoke of Heat-Not-Burn Tobacco Product for HPLC AnalysisErrors arising from the DNPH approach commonly used to analyze carbonyl compounds in smoke from heat-not-burn (HNB) tobacco can be avoided by using the approach described in this study.
Latest:
Microbial Metabolomics Based on GC Analysis as the New Avenue in Understanding the Complex Systems of Microorganisms: Current Challenges on Sample PreparationNew approaches to sample preparation are being developed for microbial metabolomics to address the inherent complexity of this type of analysis.
Latest:
A Suitable Therapeutic Drug Monitoring Method for Amoxicillin in Plasma by High Performance Liquid Chromatography–UV (HPLC–UV) in NeonatesThis simple, rapid, and accurate HPLC-UV method can facilitate routine therapeutic drug monitoring in neonates.
Latest:
Liquid Chromatography–Mass Spectrometry Analysis of Hop-Derived Humulone and Isohumulone Constituents in Beer: The Bitter Truth of Hops Utilization During BrewingA rapid LC–MS method using ESI coupled with SIM-MS for analysis of humulone and isohumulone content in beer is described.
