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The Application Notebook-02-01-2013

The Application Notebook

Effects of Particle Size and Column Length on IEX Charge Variant Analysis of Monoclonal Antibodies

February 01, 2013

Medical/Biological

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A commercially available monoclonal antibody was analyzed via two different particle sizes (5 ?m and 3 ?m) and column lengths (100 mm and 30 mm) to investigate their effects on resolution and the ability to increase sample throughput.

Determination of Phthalates Leached from Toys into Artificial Saliva

February 01, 2013

Environmental

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Phthalates are plasticizers that are added to plastics to make them softer and more flexible. Plastics that contain phthalates are commonly used in the manufacturing of toys. Since phthalates are not permanently bonded to plastics, they can be released from toys through touching, licking, and chewing.

New Generation Monolithic Silica Columns for Fast, High Resolution Drug Separations without High Pressures

February 01, 2013

Pharmaceutical/Drug Discovery

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Using a novel new-design monolithic silica column (Chromolith? HighResolution RP-18e), fast, high resolution separations were achieved for various drug mixtures without the high pressures characteristic of modern particulate technology.

Direct Analysis of Drugs in Biofluids

February 01, 2013

Medical/Biological

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Sample pretreatment is often a time consuming and labor-intensive step which can be a restricting factor for the rapid analysis. This is especially true for the analysis of drugs in biofluids containing many interfering matrices.

An MDL Study Using Automated Solid Phase Extraction of Semi-Volatile Organic Compounds (AB 8270 SIM ) in Water

February 01, 2013

Environmental

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EPA Method 8270 is used to determine the concentration of semi-volatile organic compounds in extracts prepared from solid waste matrices such as soils, air sampling media, and water samples. Extractions from water samples can be particularly difficult and time consuming due to the problematic analytes that need to be extracted, concentrated, and analyzed.

Direct Analysis Serum Lipidomics Using High Performance Time of Flight Mass Spectrometry — High Resolution, Accurate Mass, and Zucker Rats

February 01, 2013

Medical/Biological

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Polar lipids and other metabolites extracted from plasma from lean, diabetic, and obese Zucker rats were analyzed by flow injection using high resolution time of flight mass spectrometry. Metabolites were confidently identified and differences in the metabolite profiles determined.

Isolation and LC–MS Characterization of Illicit Bath Salts in Urine

February 01, 2013

Medical/Biological

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In this article, the analysis of bath salts (phenethylamine and cathionone compounds) from urine samples is demonstrated using polymeric solid phase extraction (SPE) sample preparation followed by HILIC chromatographic analysis with TOF-MS detection. HILIC chromatographic conditions on the Ascentis? Express HILIC phase are used for fast, high-resolution separation of nine synthetic bath salts.

Using an In-House Hydrogen Generator to Replace He Carrier Gas for GC

February 01, 2013

General

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H2 is commonly used as GC carrier gas as the cost of He is increasing and its availability is decreasing. In addition to the cost/availability issues, H2 can provide more rapid separations at lower temperatures than He and provides longer column life. H2 gas is obtained by the electrolysis of water and many laboratories supply it to the GC via an in-house H2 generator.

Hyaluronic Acid (Polysaccharides)

February 01, 2013

Medical/Biological

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Hyaluronic acid (HA) is a ubiquitous, very high molar mass polysaccharide that has been of particular importance in opthalmic surgery. HA acts as a molecular "shock-absorber" and stabilizer for cells and its visco-elastic properties are valuable for separating tissue and maintaining shape.

Improvement in LC–MS-MS Analysis of Vitamin D Metabolites in Serum Using Column Selectivity and Effective Sample Prep

February 01, 2013

Medical/Biological

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LC–MS-MS has become an important tool to assess an individual's vitamin D status. Chromatographic resolution of the various homologs of vitamin D2 is necessary for accurate quantitation since several key metabolites are isobaric and not distinguishable by MS alone.

Effects of Small Particle Size on SEC Characterization of Monoclonal Antibodies

February 01, 2013

Medical/Biological

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A commercially available monoclonal antibody was analyzed via three different particle sizes (5 ?m, 3 ?m, and 2 ?m) to investigate the effects of particle size on resolution and the ability to increase sample throughput.

Accurate Mass Screening for Pesticide Residue Testing

February 01, 2013

Environmental

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The update to the SANCO guidelines in 2009 recognized the power and potential of TOF mass spectrometry for pesticide screening applications. When compared with historical screening tools (such as the use of triple-quad mass spectrometry), high performance accurate mass TOF systems offer several advantages for screening applications.

Introducing the Hamilton PRP-C18 High-Efficiency Separations at Any pH

February 01, 2013

General

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The Hamilton PRP-C18 is a new column designed to provide high-efficiency reversed phase separations over an extended column life in nearly any mobile phase or pH. The rigid stationary phase has excellent mechanical and thermal stability (> 100 ?C), does not experience shrinkage or swelling and is completely inert to most conditions commonly encountered in reversed phase chromatography. In this study the pH stability of the PRP-C18 is evaluated. Even after prolonged exposure to concentrated (1 molar) NaOH and H2SO4 there was no measurable deterioration in performance.

Vol 31 No 2 LCGC North America February 2013 Application Notebook PDF

February 01, 2013

Issue PDF

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Click the title above to open the LCGC North America February 2013 Application Notebook, Vol 31 No s2, in an interactive PDF format.