The Application Notebook-02-01-2022

Characterization of mAb Glycoforms on FcγRIIIA-Ligand by Analytical and Semi-Preparative Chromatography

February 01, 2022

40

S2

To monitor FcR receptor binding, a recombinant FcγRIIIa-ligand has been commercialized into TSKgel FcR-IIIA high-performance affinity chromatography (AFC) columns that separate antibodies based on affinity differences to FcγRIIIa.

Analysis of Long-Chain Oligo-DNA with the Shodex™ VN-50 2D HILIC Column

February 01, 2022

40

S2

This technical article outlines the analysis of long-chain oligonucleotides utilizing a simple eluent containing acetonitrile with a relatively low level of salt and no ion-pair reagent.

PRP C-18, Unlock the Spice: Isolation of Garlic Components by Reversed-Phase HPLC

February 01, 2022

40

S2

With a multitude of health applications, garlic separations appear to be a necessary quest.

Analysis of 14 Cannabinoids

February 01, 2022

40

S2

In order to evaluate and test the robustness of cannabinoid analysis using the miniLC, a method was chosen from the literature and compared to data from the miniLC system. Comparison of retention time of standards to published values indicates the miniLC provides an excellent alternative with comparable results to a more expensive, complicated liquid chromatography system

ChloroFiltr®: A Novel Sorbent for Chlorophyll Removal Using QuEChERS

February 01, 2022

40

S2

Spinach and other highly pigmented vegetables contain chlorophylls, carotenoids, xanthophylls, and anthocyanins. Chlorophylls have the greatest adverse effect on gas chromatography systems due to their non-volatile characteristics. This QuEChERS procedure uses ChloroFiltr to significantly reduce chlorophylls without sacrificing the recoveries of planar pesticides.