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The Application Notebook-06-01-2020
MilliporeSigma - Online SPE and LC–MS Analysis of Thyroid Hormones in Human Serum
June 01, 2020
Medical/Biological
38
6
Restek - Analysis of Fentanyl and Its Analogues in Human Urine by LC–MS/MS
June 01, 2020
Medical/Biological
38
6
Abuse of synthetic opioid prescription painkillers such as fentanyl, along with a rapidly growing list of illicit analogues, is a significant public health problem. In this study, we developed a simple dilute-and-shoot method that provides a fast 3.5-min analysis of fentanyl and related compounds (norfentanyl, acetyl fentanyl, alfentanil, butyryl fentanyl, carfentanil, remifentanil, and sufentanil) in human urine by liquid chromatography–tandem mass spectrometry (LC–MS/ MS) using a Raptor Biphenyl column.
Waters - Size-Exclusion Chromatography for the Impurity Analysis of Adeno-Associated Virus Serotypes
June 01, 2020
Pharmaceutical/Drug Discovery
38
6
Monitoring the size heterogeneity of AAV-based gene therapy therapeutics is potentially important to ensure consistent product quality and efficacy. We demonstrate that the levels of both high-molecular weight (HMW) and low-molecular weight (LMW) impurities in AAV capsid preparations can be separated on a Waters 450 Å Protein BEH SEC column for a series of AAV serotypes.
Pickering Laboratories - Amino Acid Analysis by European Pharmacopeia to Support Coronavirus Research
June 01, 2020
Pharma/Drug Discovery
38
6
Pharmaceutical companies worldwide are currently searching for vaccines, therapeutic agents, and prophylactics to combat COVID-19. The focus on biologic drugs during the current pandemic highlights the crucial role amino acids analysis plays in research and production of pharmaceuticals.
Waters - An Anion-Exchange Chromatography Method for Monitoring Empty Capsid Content in Adeno-Associated Virus Serotype AAV8
June 01, 2020
Pharma/Drug Discovery
38
6
The single-stranded DNA (ssDNA) content of AAV capsids in AAV-based gene therapy preparations impacts the efficacy of this treatment modality. We demonstrate that AAV8 capsids without and with full length ssDNA can be separated and their relative abundances determined on a Waters Protein-Pak Hi Res Q strong anion-exchange column.