June 2022 | LCGC Europe

Supercritical Fluid Chromatography Coupled with Drift Time Ion Mobility Quadrupole Time-of-Flight Mass Spectrometry as a Tool for Lipid Characterization of HepG2 Cells

June 01, 2022

Peer Reviewed

35

6

Lipids in SFC are mainly separated according to their lipid classes, which simplifies quantification with standards. In this study, SFC coupled with drift time IM‑QTOF-MS was used to characterize the HepG2 lipidome.

Dealing with Wandering First-Dimension Peaks in 2D-LC Separations

June 01, 2022

LC Troubleshooting

35

6

Retention time fluctuations are challenging to deal with, especially in two-dimensional liquid chromatography (2D‑LC) separations. Here, two approaches are presented that can help navigate this challenge.

Column Overload in Porous Layer Open Tubular (PLOT) Columns

June 01, 2022

Column Watch

35

6

The basic types of column overload (volume and mass) and the variables that most affect the sample loading capacity in PLOT column chromatography are examined.

Diving into the Structural Details of In Vitro Transcribed mRNA Using Liquid Chromatography–Mass Spectrometry-Based Oligonucleotide Profiling

June 01, 2022

Biopharmaceutical Perspectives

35

6

An overview of mRNA-based vaccine production and a discussion on the methods that are currently in use to check the quality of these vaccines.

Co-Polymer Sequence Determination Using SEC Combined with Py-GC

June 01, 2022

35

6

LCGC Europe spoke to Wouter Knol from the University of Amsterdam, The Netherlands, about co‑polymers, the difficulties surrounding their analysis, and what pyrolysis-gas chromatography (Py‑GC) offers in comparison to other techniques when analyzing co-polymers and their sequence.

10th International Symposium on Recent Advances in Food Analysis (RAFA 2022)

June 01, 2022

Events

35

6

The 10th International Symposium on Recent Advances in Food Analysis (RAFA 2022) will take place in Prague, Czech Republic, on 6–9 September 2022.