LCGC North America-01-01-21

LC Troubleshooting Odds and Ends from 2020

January 01, 2021

LC Troubleshooting

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Sometimes our approach to troubleshooting specific problems has to change in response to changes in high performance liquid chromatography (HPLC) technology over time. In this installment, we discuss changes in technologies for mobile-phase degassing, silica-based stationary phases, and models for reversed-phase selectivity.

Retention Loss of Reversed-Phase Columns Using Highly Aqueous Mobile Phases: Fundamentals, Mechanism, and Practical Solutions

January 01, 2021

Peer-Reviewed Article

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We investigate a phenomenon observed by many practitioners of reversed- phase LC: a dramatic loss in retention volume or time after the column flow is stopped and resumed when using highly aqueous mobile phases with certain columns.

Turning Up the Heat: The Effect of Temperature on Analytical Extractions

January 01, 2021

Sample Prep Perspectives

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Temperature is frequently employed to enhance analytical extractions, especially with solid samples or volatile analytes. Using temperature to enhance analytical extractions continues today with more recently developed techniques, which we explore.

What Chromatograms Can Teach Us About Our Analytes

January 01, 2021

GC Connections

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For GC, how do data systems both assist and hinder us in obtaining maximum information from chromatograms? We explain how a chromatogram can provide a wealth of information about an individual analyte in a sample, about the sample itself, and about how well a GC instrument is performing.

The Multi-Attribute Method (MAM) for the Characterization of Biopharmaceuticals

January 01, 2021

Focus on Biopharmaceutical Analysis

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New analytical workflows are needed to address the advances in biopharmaceutical product composition. A description of the multi-attribute method (MAM) is given, which has been developed to monitor critical quality attributes (CQAs) simultaneously and directly.