LCGC North America-09-01-2016

LCGC North America

Statistics for Analysts Who Hate Statistics, Part II: Linear Regression and Quantitative Structure–Retention Relationships

September 01, 2016

Special Feature

34

9

This is part two of a series of tutorials that explain, in the simplest manner, how statistics can be useful, even to chromatographers who normally find statistics difficult, with a minimal understanding of its features. This part explains linear regressions.

Practical Considerations of Capillary Electrophoresis–Mass Spectrometry for Analysis of Biotherapeutics

September 01, 2016

Biotechnology Today

34

9

In this article, we discuss the use of CE-MS (sheath flow interface) for analysis of intact proteins as well as of protein digests. We discuss the unique aspects that the user needs to be aware of while testing biotherapeutics versus small molecule drugs. We also highlight that the optimization of CE and MS parameters together result in the creation of a more robust and reproducible protein analysis approach. Finally, we list some of the most common errors that are likely to occur during CE-MS analysis and suggest ways to overcome them.

The Hydrophobic-Subtraction Model for Reversed-Phase Liquid Chromatography: A Reprise

September 01, 2016

Peer-Reviewed Article

34

9

Reversed-phase liquid chromatographic columns can be compared quantitatively for differences in selectivity by means of the hydrophobic-subtraction model. This allows selection of columns that are either equivalent or different in selectivity. The present paper both presents a summary of this approach and shows in detail how to use it in practice.

Highlights from HPLC 2016

September 01, 2016

Column Watch

34

9

HPLC 2016, chaired by Professor Robert Kennedy, was held June 19–24 in San Francisco, California, at the Marriott San Francisco Marquis. This installment of "Column Watch" covers some of the highlights observed at the symposium including stationary-phase developments, particle technology, and areas of growing application of HPLC. In addition, trends and perspectives on future developments in HPLC noted from the conference are presented.

What HPLC Operators Need to Know, Part II: Instrument and Detector Settings

September 01, 2016

The Essentials

34

9

A practical step-by-step guide to setting up an HPLC instrument with hints and tips to avoid common pitfalls.

Vol 34 No 9 LCGC North America September 2016 Regular Issue PDF

September 01, 2016

Issue PDF

34

9

Click the title above to open the LCGC North America September 2016 regular issue, Vol 34 No 9, in an interactive PDF format.