Special Issues-12-01-2015

SilcoTek® Corporation offers custom coatings to chromatographers, and engineers worldwide. Our patented CVD coating technologies improve analytical reliability, reduce errors, and increase your bottom line. SilcoTek® coating technologies are utilized in diverse applications and industry segments worldwide. Whether it's an inert flowpath, improved corrosion resistance, bio-inertness, or improved durability; our coatings are here to expand the material limits of your products.

SPEX CertiPrep supplies CRMs to laboratories worldwide in the following markets: R&D, Academia, Agriculture, Food and Beverage, Environmental, Waste Treatment, Consumer Safety, Government and Industrial Testing, Clinical & Pharmaceutical research, Energy, Petrochemical & Petroleum industries, and the new field of Cannabis testing.

SiliCycle® Inc. is a worldwide leader in the development, manufacturing, and commercialization of high value silica-based and specialty products for chromatography, analytical and organic chemistry. We are at the forefront of the chromatography industry, owing to the extraordinary purity of our silica gels and sorbents, customizable to meet the specific requirements of scientists worldwide. With our multi-ton manufacturing capacity, we are your partner of choice for all your analysis, metal removal, catalysis, synthesis, and purification requirements.

UCT is headquartered in Bristol, Pennsylvania, with a research facility in Scranton, Pennsylvania. Manufacturing and distribution sites are in Lewistown, Pennsylvania and Wexford, Ireland. UCT is represented worldwide by over 47 partners and distributors.

Supelco's HPLC product offerings include Titan™ UHPLC columns, Ascentis® Express Fused-Core® HPLC columns, plus a new Fused-Core BIOshell™ column that improves protein and peptide analyses by reversed phase; the same way Ascentis Express columns advance the analysis of small molecular weight compounds.

Thermo Fisher Scientific is the world leader in serving science. Our mission is to enable our customers to make the world healthier, cleaner, and safer. We help our customers accelerate life sciences research, solve complex analytical challenges, improve patient diagnostics, and increase laboratory productivity. Through our four premier brands Thermo Scientific, Life Technologies, Fisher Scientific, and Unity Lab Services we offer an unmatched combination of innovative technologies, purchasing convenience, and comprehensive support.

Wyatt's products are the most widely used multi-angle light scattering (MALS) detectors in the world. They are employed in thousands of laboratories including, but not limited to, companies involved in biotechnology, pharmaceutical development, and chemical and petrochemical research, in addition to government national labs and academic facilities.

Waters operates cGMP, ISO 13485:2003, and ISO 9001-2008-certified manufacturing plants in Milford, Massachusetts (instruments and parts); Wilmslow, UK (mass spectrometers); Taunton, Massachusetts (chemistries); and Wexford, Ireland (chemistries and mass spectrometers). Waters' Milford facility is also approved for Part 1, Canadian Medical Device Regulations (CMDR). Waters operates 23 United States facilities and 78 international facilities, including field offices.

Tosoh Bioscience LLC is located in King of Prussia, Pennsylvania. Supply chain offices are in Grove City, Ohio. European operations are in Stuttgart, Germany. Asia is served by Tosoh Corporation in Tokyo, Japan; Shanghai, China; and Singapore. Products are manufactured by Tosoh Corporation in Japan.

MicroLiter Analytical Supplies, Inc., A WHEATON® Brand, was founded in 1996 to supply autosampler vials and consumables for the chromatography autosampler market, including consumable vials, closures with septa, inserts, and microplates. MicroLiter offers Certified Class 10,000 clean room packaged autosampler vials and inserts, ensuring minimal particulates from product packaging. A comprehensive offering of crimp seals, screw thread, and snap caps, with a variety of septa configurations, are packaged in tubes to allow the use of a bench top dispenser. This unique dispenser minimizes environmental contamination to the closures once the packaging is opened. The MicroLiter Plate Sampling System (MPSS) is a patented system of microplates with glass inserts and re-sealable closures. The MPSS allows our customers to prep samples using liquid handlers in a 96-well format and transfer samples with minimal amounts of human interaction to the analytical instrument.

Pharmaceutical, nutraceutical, basic science research, antibody purification, food and cosmetics, chemical manufacturing (pesticides, polymers), biological research (phosphopeptide enrichment), and chiral separation.

The majority of the samples in this study and other studies show that a majority of compounds can be separated by the use of the three most common alcohols: IPA, CH3OH, and ethanol. Solubility becomes a crucial factor in preparative chromatography. Combinations of solvents can be used to improve solubility when the chosen co-solvent has poor solubility of the compound in question.

Comprehensive 2D-LC enables the analysis and quantification of purine alkaloids and catechins in green and black tea. As expected, green tea contained higher amounts of the catechins epigallocatechin gallate and epicatechin than black tea.

This automated SPE methodology is a solution that will reduce analyst labor, solvent usage, and turn-around-time while maintaining the high quality results required within today's laboratories.

TSKgel UP-SW3000 columns are 2 µm SEC columns designed for the analysis of monoclonal antibodies and other biopharma products. Higher resolution can be achieved for the separation of antibody monomers, dimers, and higher order aggregates with a TSKgel UP-SW3000 column compared to a competitor UHPLC column. The TSKgel UP-SW3000 column provided excellent reproducibility for the peak parameters of retention time, asymmetry, and column efficiency. As demonstrated by the %RSD values, injection-to-injection reproducibility was superior to the competitor column.

Homogenize 2 g of dry tea leaves with 25 mL of Li220 for 5 min. Centrifuge and filter through 0.45 µm nylon filter.

TSKgel UP-SW3000 columns are 2 µm SEC columns designed for the analysis of monoclonal antibodies and other biopharma products. Higher resolution can be achieved for the separation of antibody monomers, dimers, and higher order aggregates with a TSKgel UP-SW3000 column compared to a competitor UHPLC column. The TSKgel UP-SW3000 column provided excellent reproducibility for the peak parameters of retention time, asymmetry, and column efficiency. As demonstrated by the %RSD values, injection-to-injection reproducibility was superior to the competitor column.

Following sampling, tubes were transferred to a Markes UNITY™ thermal desorption system paired with an Agilent 7890B GC that was coupled to an Agilent 5977A MS detector. The UNITY™ system and GC–MS parameters are presented in Table I and Table II, respectively.