LCGC UST
News|Videos|October 22, 2025 (Updated: October 23, 2025)

Analyzing Oligonucleotides With Higher Order Structures

The panel explore the unique challenges associated with analysing double- stranded oligonucleotide products and oligonucleotides with higher-order structures.

When working with oligonucleotides, complexity can significantly impact performance and reliability. Double-stranded products and sequences with higher-order structures present unique challenges in synthesis, handling, and application. These structural features can affect stability, hybridization, and even downstream results. In this video, we explore the specific hurdles researchers face when working with these types of oligonucleotides—and how to overcome them. Catch the discussion in the video above.

Related to this article

Best of the Week: From Olive Waste to mRNA Vaccines
This week's Chromatography Online highlights include olive waste antioxidant recovery, food and tea analysis, EV isolation, mRNA vaccine purification, perfusion chromatography for oligonucleotides, mass spectrometry in teaching and career advice from “Women in Chromatography.”