The Rise of Ion-Pair Reversed-Phase HPLC for Oligonucleotides

Few techniques have gained as much traction for oligonucleotide separation as ion-pair reversed-phase liquid chromatography (IP-RP-HPLC). But what exactly led to its rise in popularity and widespread use in both research and biopharmaceutical settings? In this video, the panelists discuss the factors behind the adoption of IP-RPHPLC for oligonucleotide separations. From its robust compatibility with mass spectrometry to its impressive resolution and reproducibility, the method has become a cornerstone in oligonucleotide analysis. Watch the discussion in the video above.