The Role of IEX and HILIC for Oligonucleotide Analysis

The panelists explores how ion-exchange chromatography (IEX) and hydrophilic interaction liquid chromatography (HILIC) compare to ion-pair reversed-phase high performance liqud chromatography (IP-RP-HPLC) for analyzing polar and charged compounds. They highlight the strengths and limitations of each technique, including selectivity, compatibility with mass spectrometry, and retention behavior. IEX is praised for its effectiveness with charged species, while HILIC offers advantages for highly polar analytes and better MS compatibility than ion-pair RPHPLC. The video discusses the growing interest in replacing IP-RP-HPLC due to challenges such as poor MS sensitivity and method reproducibility. Watch the video above.