John Dolan has been writing "LC Troubleshooting" for 

 for over 20 years. One of the industry's most respected professionals, John is currently the Vice President of BASi Northwest Laboratory, McMinnville, Oregon and a principal instructor for LC Resources, Walnut Creek, California. He is also a member of 

Liquid Chromatography (LC/HPLC)

HPLC

Column: Column Watch

Columns

LCGC Europe-06-01-2005

LCGC Europe

In the last part of this series, the authors provide a technical review of the means for method adjustment and the pertinent regulations concerning the required validation of adjusted methods.

Adjusting Conditions for a Routine Reversed-Phase HPLC Assay, Part 2: Changing Separation Conditions