John Dolan has been writing "LC Troubleshooting" for 

 for over 25 years. One of the industry's most respected professionals, John is currently a principal instructor for LC Resources, Walnut Creek, California He is also a member of 

Ron Majors, editor of "Column Watch" and "Sample Prep Perspectives," has been with 

 for over 26 years. Currently a senior scientist with Agilent Technologies, Wilmington, Delaware, Ron is known industry-wide as one of the premier chromatography experts in the field. He is also a member of 

Liquid Chromatography (LC/HPLC)

HPLC

Column: Column Watch

Columns

LCGC Europe-06-01-2005

LCGC Europe

In the last part of this series, the authors provide a technical review of the means for method adjustment and the pertinent regulations concerning the required validation of adjusted methods.

Adjusting Conditions for a Routine Reversed-Phase HPLC Assay, Part 2: Changing Separation Conditions