|Articles|December 1, 2001

LCGC North America

  • LCGC North America-12-01-2001
  • Volume 19
  • Issue 12

New Developments in the Application of Monolithic HPLC Columns

The guest authors discuss the use of monolithic silica columns in high-throughput HPLC, including developments and applications in combinatorial chemistry. They also explain performance characteristics for these columns and provide caveats about their effective usage.

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Product Showcase

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2001 Index of Authors and Subjects

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Chromatography Detects Toxins in Tea Samples
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Figure 2. Split injection used to measure triplicate test of MTBE, hexane, o-xylene, and 1-methylnaphthalene comparing different solvents (methanol, methylene chloride) and column insertion distances starting with 0.5 mm, 5.0 mm, 10.0 mm, 15 mm, and 20.0 mm. Methanol had the best performance at the 5.0 mm insertion distance and methylene chloride looked slightly better at the 0.5 mm. We would still recommend not going below the 5.0 mm manufacturer recommended insertion distance.
Revisiting insertion distance for methanol and methylene chloride in GC analysis and confirming that insertion depth strongly affects response and reproducibility.