One- and Two-Dimensional LC/MS/MS Analysis of Arabidopsis thaliana Proteome
This application note compares a 1-D and 2-D HPLC separation coupled to MS for proteome analysis and finds that the 2-D method identifies 30% more proteins. (Grace Vydac)
Articles in this issue
Determination of Triglyceride Composition of Vegetable Oils Using High Performance Liquid Chromatography and Evaporative Light Scattering Detection
One- and Two-Dimensional LC/MS/MS Analysis of Arabidopsis thaliana Proteome
Extraction of Low Concentration Levels of 6-MAM from Urine with a Mixed-Mode SPE Sorbent — Strata™ Screen-C
Analysis of U.S. EPA Method 8270D — Semivolatile Organic Compounds by GC/MS
Hemoglobin Characterization
"Green" Analysis of Diet Soft Drinks Containing Caffeine and Aspartame
Carbamate Pesticide Analysis: Convergence of Micro Postcolumn Derivatization and LC–MS
A Reversed-Phase HPLC Column for the Separation and Characterization of Peptides Using LC-UV-MS
Mobile Phase Optimization for the Analysis of an Antibody-Based Fusion Protein and Aggregates Using a TSKgel Super SW3000 Size-Exclusion Column
The Determination of Glycerol in Wine by HPLC with Low Temperature Evaporative Light Scattering Detection
Morphine
New LC Phase for the Separation of Phospholipids: Pholipidec®
High Throughput Screening — LC/MS with ELS Detection
Improved Peak Resolution and Shape for Antidepressants on a New Silica-Based Reversed-Phase Column
EDTA
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Why is Open-Access Hyphenation Important for Chromatographers?
October 4th 2023Article
At HPLC 2023, John Langley, a professor at the University of Southampton in Southampton, UK, presented a talk called "(R)evolution of Hyphenations, Through Open-Access RP-HPLC–MS, Open-Access UHPSFC–MS and 2D-LC–IMS-MS in Academia". He spoke with LCGC on the importance of open-access hyphenation, and how to implement it in your research.
