This application note compares a 1-D and 2-D HPLC separation coupled to MS for proteome analysis and finds that the 2-D method identifies 30% more proteins. (Grace Vydac)
Next Generation Peak Fitting for Separations
July 9th 2024Separation scientists frequently encounter critical pairs that are difficult to separate in a complex mixture. To save time and expensive solvents, an effective alternative to conventional screening protocols or mathematical peak width reduction is called iterative curve fitting.
Eyes on the Prize: Overcoming Uncertainty to Realize the Power of 2D-LC Separations
June 10th 2024In this month’s column, I highlight some of the primary considerations we face in method development and point to resources that can help users overcome uncertainty and develop highly effective 2D-LC methods.
Why is Open-Access Hyphenation Important for Chromatographers?
October 4th 2023At HPLC 2023, John Langley, a professor at the University of Southampton in Southampton, UK, presented a talk called "(R)evolution of Hyphenations, Through Open-Access RP-HPLC–MS, Open-Access UHPSFC–MS and 2D-LC–IMS-MS in Academia". He spoke with LCGC on the importance of open-access hyphenation, and how to implement it in your research.