The efficiencies of microbore-2 columns, which are prepared from blanks that have a wide variety of inner surface roughness, drop sharply when the size of individual surface roughness features approaches the particle size of the packing material. The results suggest that two categories of packed column structure relate to the surface features and yield high and low efficiency columns. This installment of "Column Watch" discusses this conclusion in terms of the stability of an agglomerated layer of packing particles on the blank wall when subjected to shear forces during column packing.
LCGC 18(2), 106–117 (2000).HPLC 2025 Preview: The Present and Future of Automation in Analytical Laboratories
May 22nd 2025Analytical laboratories are undergoing a fundamental transformation. In the face of increasing sample volumes, growing regulatory requirements, and the rising demand for faster, more precise, and cost-efficient analysis, optimizing laboratory processes is becoming a central focus. Automation technologies offer promising solutions in this regard. Recently, they have evolved from isolated solutions to comprehensive systems that permeate nearly all areas of laboratory practice. This development not only opens up new opportunities in terms of efficiency, data quality, and scalability but also brings technical, organizational, and personnel challenges. To successfully address these, strategic approaches are needed that consider both the technological and human dimensions of the transformation.
Quantifying Isavuconazole in Dried Blood Spots Using HPLC
May 21st 2025Isavuconazole, an antimycotic agent used to treat fungal infections, can typically be found during dried blood spot sampling. However, there are obstacles that keep it from being an ideal approach for properly determining the drug’s presence.
HPLC 2025 Preview: On The Road With Your Chromatograph?
Published: May 21st 2025 | Updated: May 21st 2025Brett Paull from the University of Tasmania, Tasmania and his team describe the latest development in portable LC instruments and their experience of taking portable systems out to the field.