Tips&Tricks: GPC/SEC What is the Best Detector Sequence?

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The Column

The Column, The Column-10-04-2012, Volume 0, Issue 0
Pages: 9–12

GPC/SEC (gel permeation chromatography/size exclusion chromatography) methods generally contain multiple detectors in order to gather more information about the molecular structure of macromolecules.1. Up until now, however, the practice and the impact of detector combination has not been discussed.

GPC/SEC (gel permeation chromatography/size exclusion chromatography) methods generally contain multiple detectors in order to gather more information about the molecular structure of macromolecules.1 Such detectors include: concentration detectors [refractive index (RI) and ultraviolet (UV) are the most commonly used]; molar mass sensitive detectors (light scattering, viscometers, mass spectrometers); and information-rich detectors [Fourier transform infrared (FTIR), and nuclear magnetic resonance (NMR)]. Over the years the information and results available from different detector combinations have been the subject of various Tips & Tricks articles. Up until now, however, the practice and the impact of detector combination has not been discussed.

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