Tips&Tricks: GPC/SEC What is the Best Detector Sequence?
GPC/SEC (gel permeation chromatography/size exclusion chromatography) methods generally contain multiple detectors in order to gather more information about the molecular structure of macromolecules.1. Up until now, however, the practice and the impact of detector combination has not been discussed.
GPC/SEC (gel permeation chromatography/size exclusion chromatography) methods generally contain multiple detectors in order to gather more information about the molecular structure of macromolecules.1 Such detectors include: concentration detectors [refractive index (RI) and ultraviolet (UV) are the most commonly used]; molar mass sensitive detectors (light scattering, viscometers, mass spectrometers); and information-rich detectors [Fourier transform infrared (FTIR), and nuclear magnetic resonance (NMR)]. Over the years the information and results available from different detector combinations have been the subject of various Tips & Tricks articles. Up until now, however, the practice and the impact of detector combination has not been discussed.
LC–MS/MS-Based System Used to Profile Ceramide Reactions to Diseases
April 26th 2024Scientists from the University of Córdoba in Córdoba, Spain recently used liquid chromatography–tandem mass spectrometry (LC–MS/MS) to comprehensively profile human ceramides to determine their reactions to diseases.
High-Throughput 4D TIMS Method Accelerates Lipidomics Analysis
April 25th 2024Ultrahigh-pressure liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-HRMS) had been previously proposed for untargeted lipidomics analysis, but this updated approach was reported by the authors to reduce run time to 4 min.
