Agilent Technologies, Inc.

Webinar Date/Time: Wed, Aug 23, 2023 11:00 AM EDT

Webinar Date/Time: Thu, Aug 31, 2023 2:00 PM EDT

In this study, the automatized Agilent 1290 Infinity II system was used for purification of native single stranded oligonucleotides (ONs). The separations were performed with IP-RPLC using dibutylamine (DBA) and tris(hydroxymethyl) aminomethane (TRIS). The combined separation methods with instrumental setup with columns, allowed for the target compound to be collected faster and with higher purity, compared to conventional methods.

This poster evaluates the performance of the end-to-end workflow for the extraction, screening, identification, and quantitation of over 80 sphingolipids from 10 classes in plasma, a method using Agilent 1290 Infinity II LC coupled to an Agilent 6495 triple quadrupole LC/MS system.

This poster exhibits how combining XF and LC/MS technologies can provide deeper insight into the cellular and molecular metabolic response to drug treatment for cancer research.

This poster illustrates the outstanding quantification performance for large panel pesticide screening analysis in a known difficult matrix such as cinnamon, using a novel LC/TQ platform, including the Agilent 1290 Infinity II Bio LC system coupled to the 6495 Triple Quadrupole LC/MS with the Jet Stream Technology Ion Source (AJS).

This study assesses a workflow developed for PFAS analysis in wastewater, based on the existing EPA draft Method 1633 with additional PFAS of varying size and functional group. This workflow contains off-line solid phase extraction (SPE) and a clean-up step, followed by LC-MS/MS analysis, and automatic reporting using SLIMS. The workflow demonstrates a reliable solution for the targeted analysis of PFAS in complex matrices with high robustness.

This study demonstrates the 6495 LC/TQ (G6495D) is suitable for comprehensive protein quantification assays in complex matrix.

This poster demonstrates how the HILIC polar metabolite workflow is enhanced with the new 4th generation 6495 LC/TQ, and robust quantitation is enabled with new 13C metabolite transitions.

This poster shows that over 200 pesticides screened for using a DIA mode of acquisition from 0.625 ppb to 100 ppb​,​ good Mass Accuracy, Low RSD’s, and Scored Coelution of Fragment Ions​ are demonstrated.

This poster demonstrates that the Agilent 6495 triple quadrupole LC/MS system shows increased analytical sensitivity of nitrosamine impurities at the low concentration levels specified by regulatory requirements.

In an ever-evolving cannabis industry where regulatory compliance and accurate testing are paramount, staying ahead requires a deep understanding of how to build an efficient testing lab that meets compliance standards:

Webinar Date/Time: Wed, Sep 13, 2023 11:00 AM EDT

This short video provides an overview of a complete, end-to-end oligonucleotide workflow solutions

Characterization of oligonucleotides requires robust analytical instrumentation and methods as well as ease-of-use data analysis tools. Biocompatibility mitigates non-specific sample binding to flow path and it ensures the integrity of biomolecules and robustness of the system. In this study, two workflows, the Target Plus Impurities (TPI) and Sequence Confirmation workflows in Agilent MassHunter BioConfirm software, were carried out to characterize two oligonucleotide samples.

In this application note, the determination of oligo sequence confirmation using HILIC LC and high-resolution MS/MS data is described. As with the previous studies, an InfinityLab Poroshell 120 HILIC-Z column was used along with an Agilent 6545XT AdvanceBio LC/Q-TOF mass spectrometer.

In this application note, LC separation and MS1 mass identification of a variety of oligos without the use of ion‑pairing reagents is demonstrated. The LC separation allows subsequent positive mode use with little to no flushing or hardware changes. This HILIC-based method uses an Agilent InfintyLab Poroshell 120 HILIC-Z column and MS-friendly ammonium acetate-based mobile phases. The samples were analyzed on an Agilent 1290 Infinity II LC system and a 6545XT AdvanceBio quadrupole time-of-flight mass spectrometer (LC/Q-TOF).

End-to-End Workflow Solutions for Oligonucleotide Analysis - From research discovery to production QA/QC

UV-Vis spectrophotometers have been used widely for nucleic acid quantification and quality control (QC) utilizing the fact that nucleic acids have a maximum absorbance at 260 nm (1). The concentration of nucleic acids can be easily estimated using the absorbance at 260 nm and the established absorption coefficient. Often a background correction is also performed, for example collecting a baseline using a solution containing everything but the nucleic acid or by measuring the absorbance at a wavelength that nucleic acids do not absorb. Double stranded nucleic acids are bound by hydrogen bonds between the base pairs. The temperature at which double stranded nucleic acids denature to become single stranded depends on the: – sequence and length of the nucleic acid – the pH and buffer conditions – and any mismatches in base pairs between the two strands As such, the melting temperature is very useful analytical tool and can be studied by monitoring the absorbance at 260 nm as temperature is increased or decreased. As the temperature is increased, the hydrogen bonds between the strands are broken and the double stranded nucleic acid separates into two separate strands. When the strands separate, the absorbance at 260 nm increases. The transition temperature is called “melting temperature” (Tm) (1).

Webinar Date/Time: Tue, Jun 27, 2023 11:00 AM EDT

If you are under pressure to deliver meaningful results, the new Agilent 6495 triple quadrupole LC/MS (LC/TQ) provides sensitivity without compromising robustness. Built-in instrument intelligence of the 6495 LC/TQ enhances throughput, and integrates comprehensive workflows to get your lab up and running fast, allowing even novice users to generate expert results.

To stay competitive and adjust to changing requirements in food and environmental testing, you need evolved high resolution, high mass accuracy screening instrumentation at the core of your complete workflow solution. The Revident quadrupole time-of-flight LC/MS system (LC/Q-TOF) answers these challenges by maximizing uptime and productivity with sophisticated instrument intelligence.

Webinar Date/Time: Thu, Jun 22, 2023 2:00 PM EDT

In this exclusive video, Agilent gives an inside look at the time-consuming process behind manual integration in GC/MS, and showcases its upcoming AI/ML technology and the key benefits this software can bring to GC/MS processes – learn more in June 2023.

Get ready to be inspired by a smarter lab. View the video to see a sneak peak of the new intelligent Agilent LC/MS solutions launching at ASMS 2023.

Webinar Date/Time: Tue, Jun 13, 2023 11:30 AM EDT

Time to results can be significantly reduced with the use of RapidFire high-throughput mass spectrometry (MS). With this technique, a lab’s next great innovation can be brought from conception to reality in a timely and efficient manner.