Agilent Technologies, Inc.

Automated MHC-I is associated peptide enrichment using the Agilent AssayMAP Bravo platform with large-capacity Protein A cartridges for immunopeptidomics analysis.

The AssayMAP Bravo was used to purify, digest, and clean up antibodies from 96 cell culture supernatants with high reproducibility and minimal labor prior to LC–MS.

Intact and subunit antibody mass determination was streamlined using automated purification and enzymatic cleavage and deglycosylation of immobilized antibodies.

Automated sample prep and LC–MS peptide mapping approach to characterize and quantify multiple monoclonal antibody (mAb) critical quality attributes (CQAs)

Automated phosphopeptide enrichment from cell lysates with Fe(III)-NTA enables the identification of thousands phosphopepides with high reproducibly and selectivity.

Automated phosphopeptide enrichment and ion mobility mass spectrometry (IMS-MS) enables detailed analysis of structural effects of site-specific phosphorylation.

Automated Antibody Drug Conjugate (ADC) purification and deglycosylation increases reproducibility and simplifies drug-to-antibody ratio (DAR) determination

Improved host cell protein analysis using highly reproducible and scalable automated sample digestion and fractionation ahead of LC–MS coupled with iterative MS–MS

This application note presents a reversed-phase LC method capable of separating all relevant reduction-induced LMW species of the NISTmAb.

Wednesday, March 30th, 2022 at 11am EST | 8am PST | 4pm GMT **Wednesday, March 30th, 2022 at 2pm EST | 11am PST | 7pm GMT | The objective of this workshop is to give attendees the knowledge necessary to put together a convincing business case for purchasing a mass spectrometer. This knowledge will be imparted by presenting and discussing successful and unsuccessful examples of such business cases. Throughout the course, essential business terminology will be presented and explained.

Do you struggle with sample preparation? You are not alone! Sample preparation is often labeled as the no. 1 pain for scientists around the world. Scientists try to reduce this pain by settling for a sample preparation method or product that is less than ideal, causing even more pain further down the road. But imagine if you could eliminate some of that pain by simply choosing the right sample preparation product? In this podcast, we will help dissolve the myth that sample preparation pain is unavoidable by equipping you with proper selection recommendations and tips and tricks based on what matters to you most.

Monday, February 28, 2022 at 11am EST | 8am PST | 4pm GMT **Monday, February 28, 2022 at 2pm EST | 11am PST | 7pm GMT**Tuesday, March 1, 2022 at 12pm JST | 11am CST | 3am GMT An emerging tool to tackle the complexity of biopharmaceutical analysis is multidimensional liquid chromatography (LC). This webcast will highlight the power of 2D-, 3D- and 4D-LC in hyphenation to mass spectrometry (MS) for the detailed characterization of protein biopharmaceuticals.

Purification of a single-stranded RNA oligonucleotide by ion pair reversed-phase HPLC is demonstrated, achieving > 99% pure fraction pool with a yield of > 56%.