Electron capture dissociation produces distinct fragments of amino acid side chains, enabling the identification of isomeric amino acids such as leucine and isoleucine. This application note demonstrates the isomer identification workflow for peptides and intact proteins using the new Agilent ExD cell and ExDViewer software for fragment analysis.
Chemical characterization of medical devices, including drug-device combination products, is crucial for ensuring regulatory compliance and patient safety by identifying and quantifying chemicals that may interact with the human body. This paper explores current trends, best practices, and regulatory developments in extractables and leachables (E&L) testing for medical devices.
Webinar Date/Time: Session 1: Tue, Oct 22, 2024 11:00 AM EDT Session 2: Tue, Oct 22, 2024 2:00 PM EDT Session 3: Wed, Oct 23, 2024 2:00 PM JST
Webinar Date/Time: Session 1: Tuesday, October 15th, 2024 at 11am EDT | 3pm BST | 4pm CEST Session 2: Tuesday, October 15th, 2024 at 11am PDT | 1pm CST | 2pm EDT Session 3: Wednesday, October 16th, 2024 at 10:30am IST | 1pm CST | 2pm JST
Webinar Date/Time: Tue, Oct 8, 2024 11:00 AM EDT
This technical overview compares the characterization of NISTmAb using the ProteoAnalyzer with published NIST data obtained via traditional single capillary CE-SDS technology. The results show strong correlation with the reference data, confirming that the ProteoAnalyzer is a reliable tool for analytical workflows.
This application note demonstrates that the Agilent 6546 LC/Q-TOF in Targeted MS/MS acquisition mode is suitable for meeting performance requirements in US EPA Method 533 for the quantitative analysis of PFAS
This application note demonstrates the use of FluoroMatch Suite for the analysis and identification of per- and polyfluorinated substances (PFAS) using an Agilent 1290 Infinity II LC and Agilent 6546 LC/Q-TOF mass spectrometer with iterative exclusion (IE).
This application note describes a rapid analytical method for the detection of 20 perfluoroalkyl substances (PFAS) in human hair within 10 minutes. Solid phase extraction (SPE) using the Agilent Bond Elut ENV cartridge was used to extract the sample
Webinar Date/Time: Wed, Sep 18, 2024 Session 1: 10am EDT Session 2: 2:00 PM EDT
Webinar Date/Time: Thu, Sep 19, 2024 11:00 AM EDT
This application note demonstrates the use of the Agilent 1260 Infinity II Hybrid Multisampler for the analysis of per- and polyfluorinated alkyl substances (PFAS). The Hybrid Multisampler in feed injection mode was used with optimized Feed Speed for the sample injection to trap and enrich the compounds on the column. This avoided peak broadening and breakthrough of the early-eluting polar PFAS compounds. The less polar PFAS compounds can be enriched for more sensitive quantification.
The US EPA has released interim health advisory limits (HALs) of 4 and 20 pg/L in drinking water for the PFAS perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS), respectively. This application note addresses PFAS testing at these challenging new levels in aqueous samples using optimized solid-phase sample extraction and LC/MS/MS analysis with an Agilent 1290 Infinity II liquid chromatography (LC) system coupled to an Agilent 6495C triple quadrupole (TQ) mass spectrometer.
Webinar Date/Time: Wed, Aug 28, 2024 11:00 AM EDT
Webinar Date/Time: Mon, Aug 12, 2024 9:00 AM EDT
Webinar Date/Time: Wed, Jul 17, 2024 5:00 AM EDT
Labile post-translational modifications are better preserved using ECD than conventional MS fragmentation techniques such as collision-induced dissociation (CID). Common modifications, such as glycosylation and phosphorylation, can impact the safety, efficacy, and binding activity of monoclonal antibodies but are challenging to identify. Stephen Sciuto from Agilent Technologies and colleagues analyzed tryptic digests of NIST mAb and Infliximab using an 6545XT Q-TOF mass spectrometer in ExD or CID mode. ECD provided higher sequence coverage than CID, reaching 100% for glycosylated peptides in Infliximab. The ability to pinpoint glycosylation sites using fragment ions demonstrates the power of ECD for characterizing glycopeptides.
The first reported combination of microdroplet reactions with ECD fragmentation offers a quicker way to analyze monoclonal antibodies for disease treatments. Thomas Walker from Agilent Technologies and co-workers demonstrate the use of an Agilent Jet Stream electrospray ionization source to facilitate in-spray chemical reduction and protease digestion of monoclonal antibodies. Downstream fragmentation of the microdroplet reaction products with ECD provided rapid characterization of intact antibodies in minutes. Efficient ECD fragmentation yielded rich sequence information including evidence of disulfide bond connectivity and confirmation of key sequences called complementary determining regions. These results highlight this method’s potential for fast and cost-effective antibody characterization with minimal sample preparation.
Webinar Date/Time: Session 1: Tuesday, June 18, 2024 at 10am EDT | 7am PDT Session 2: Tuesday, June 18, 2024 at 2pm EDT | 11am PDT Session 3: Wednesday, June 19, 2024 at 12pm JST | 11am CST
Peptides are versatile molecules that have a wide range of applications in biotechnology, medicine, and research. They can be used as drugs, diagnostics, biomarkers, or tools to study cellular processes. However, peptides also pose unique challenges in terms of analysis and purification due to their complexity, diversity, and sensitivity.
Webinar Date/Time: Session 1: Thursday, May 16, 2024 at 11am EDT | 8am PDT Session 2: Thursday, May 16, 2024 at 2pm EDT | 11am PDT Session 3: Thursday, May 16, 2024 at 11am AEST | 6pm PDT
Discover how to accurately analyze purity and impurities, determine sequences, and more with next-generation technologies from Agilent.
Photodegradation of plastic products is known to accelerate weathering and the resulting release of chemical additives and particles to the environment, however these processes are complex. Here, eight different plastic consumer products were leached in double-distilled water under exposure to strong ultraviolet (UV) light for ten days, and the chemical composition of the leachates was compared to their respective dark controls. The leachates and plastic particles were investigated with a wide battery of chemical-analytical tools to broadly characterize the underlying processes and possible hazards. These covered (a) metal(loid) analysis, (b) microplastic analysis, (c) analysis of >70 organic target analytes and (d) nontargeted screening of the extracts.
Microplastics are defined as small plastic particles between 1 µm and 5 mm in size. Due to poor waste management and plastic pollution, they are now documented to be ubiquitous in the environment.1,2 However, pathways of dietary exposure to microplastics are not yet fully understood. The consumption of bottled water has been calculated to increase by 7% annually, with an estimated mean total global consumption of bottled water to be 513 billion liters by 2025. This study demonstrates how the Agilent 8700 Laser Direct Infrared (LDIR) Chemical Imaging System can accurately identify and quantify the presence of microplastics in bottled drinking water. This study also shows the capability of the Agilent Cary 630 FTIR spectrometer to investigate the source of microplastic contamination.
Reports of the omnipresence of microplastics have mainly focused on their presence in the environment, but there is a growing interest in investigating the health impacts of microplastics. Many people would assume that infants' exposure to microplastics would be limited. However, infant formula was found to be a possible exposure pathway. Extracting and isolating microplastics from infant formula can be difficult due to the range of formulations, ingredients, and components, such as fats, proteins, minerals, vitamins, and sugars. This application note demonstrates the importance of quality control in microplastics analysis. The study also shows how the Agilent 8700 Laser Direct Infrared (LDIR) Chemical Imaging System can accurately identify and quantify microplastics in infant formula.
This comprehensive peer-reviewed set of more than 600 definitions covers topics of interest for the biopharmaceutical and gene therapy subjects of genetics, therapeutics, drug development, clinical medicine, and the analytical science tools used for characterization of drugs. This glossary serves as a helpful reference to both novice and advanced scientists, engineers, and business executives involved with biopharmaceuticals and gene therapy technologies. Online sources of information for topics covered in this guide are also included for additional insights.
Agilent workflow solutions, designed for the food and beverages industry, serve as a comprehensive resource for testing alternative proteins. This compendium provides 15 applications across various domains, such as taste and flavor, nutrition, food quality, and food safety leveraging advanced analytical techniques like ICP-MS, LC/MS, GC/MS, and HPLC.
Learn how PFAS, microplastics, and chromium detection in environmental samples can be greatly enhanced with the latest LC-MS, laser directed IR spectroscopy, and IC-ICP-MS technologies
