Agilent Technologies, Inc.

This application note presents the development and validation of a multiresidue method for the analysis of PFAS in fruits, vegetables, and juices.

The increasing demand for high-purity, regulatory-compliant guide RNA (gRNA) manufacturing in the CRISPR gene editing market underscores the need for advanced production capabilities.

This application note demonstrates the superior resolution and recovery of Agilent AdvanceBio SEC 500 Å columns when compared with competitor products for AAVs.

This app note describes a non ion-pairing reversed-phase LC/MS method for highly modified ASOs, DNA, sgRNA, and siRNA ranging from 14- to 103-mer oligonucleotides.

This application note demonstrates a liquid chromatography/quadrupole time-of-flight mass spectrometer (LC/Q-TOF MS) workflow for the identification and characterization of therapeutic peptides.

Webinar Date/Time: Wed, Jan 22, 2025 11:00 AM EST

Agilent and Scitara are collaborating not only to increase the efficiency of laboratory processes, but to enable customers to leverage the value of the data for various stakeholders. This paper will present an ecosystem designed to support enterprises in accelerating data workflows and improving data availability for researchers, lab analysts, and data scientists.

Webinar Dates/Times: Tuesday, December 10, 2024 7am PST | 10am EST | 3pm GMT | 4pm CET

Webinar Dates/Times: Tue, Dec 3, 2024 11:00 AM EST

Webinar Date/Time: Wed, Dec 11, 2024 11:00 AM EST

Oligonucleotides—therapeutic agents for difficult-to-treat diseases—rely on precise molecular weight confirmation to ensure quality during development. Experts from Agilent Technologies explore the challenges of liquid chromatography-mass spectrometry (LC/MS) analysis in oligonucleotide synthesis, as well as strategies for accurate deconvolution, method development, and optimal equipment selection.

Top-down fragmentation enables rapid characterization of phosphorylated proteins without extensive sample preparation and digestion. In this study, electron capture dissociation (ECD) was used to fragment proteoforms of the cell death-related protein, Bcl-xL. Using these methods, 85–90% sequence coverage was achieved for Bcl-xL proteoforms, allowing for effective localization of phosphorylation within minutes.

Enhanced antibody analysis using electron capture dissociation (ECD) allows for precise glycan localization in low-abundance glycopeptides. This study compares the fragmentation of trastuzumab tryptic digests using ECD and collision-induced dissociation (CID). While CID generates abundant glycan HexNAc ions at 204 m/z, ECD preserves the labile glycan group, enabling accurate site localization.

Electron capture dissociation produces distinct fragments of amino acid side chains, enabling the identification of isomeric amino acids such as leucine and isoleucine. This application note demonstrates the isomer identification workflow for peptides and intact proteins using the new Agilent ExD cell and ExDViewer software for fragment analysis.

Chemical characterization of medical devices, including drug-device combination products, is crucial for ensuring regulatory compliance and patient safety by identifying and quantifying chemicals that may interact with the human body. This paper explores current trends, best practices, and regulatory developments in extractables and leachables (E&L) testing for medical devices.

Webinar Date/Time: Session 1: Tue, Oct 22, 2024 11:00 AM EDT Session 2: Tue, Oct 22, 2024 2:00 PM EDT Session 3: Wed, Oct 23, 2024 2:00 PM JST

Webinar Date/Time: Session 1: Tuesday, October 15th, 2024 at 11am EDT | 3pm BST | 4pm CEST Session 2: Tuesday, October 15th, 2024 at 11am PDT | 1pm CST | 2pm EDT Session 3: Wednesday, October 16th, 2024 at 10:30am IST | 1pm CST | 2pm JST

Webinar Date/Time: Tue, Oct 8, 2024 11:00 AM EDT

This technical overview compares the characterization of NISTmAb using the ProteoAnalyzer with published NIST data obtained via traditional single capillary CE-SDS technology. The results show strong correlation with the reference data, confirming that the ProteoAnalyzer is a reliable tool for analytical workflows.

This application note demonstrates that the Agilent 6546 LC/Q-TOF in Targeted MS/MS acquisition mode is suitable for meeting performance requirements in US EPA Method 533 for the quantitative analysis of PFAS

This application note demonstrates the use of FluoroMatch Suite for the analysis and identification of per- and polyfluorinated substances (PFAS) using an Agilent 1290 Infinity II LC and Agilent 6546 LC/Q-TOF mass spectrometer with iterative exclusion (IE).

This application note describes a rapid analytical method for the detection of 20 perfluoroalkyl substances (PFAS) in human hair within 10 minutes. Solid phase extraction (SPE) using the Agilent Bond Elut ENV cartridge was used to extract the sample

Webinar Date/Time: Wed, Sep 18, 2024 Session 1: 10am EDT Session 2: 2:00 PM EDT

Webinar Date/Time: Thu, Sep 19, 2024 11:00 AM EDT

This application note demonstrates the use of the Agilent 1260 Infinity II Hybrid Multisampler for the analysis of per- and polyfluorinated alkyl substances (PFAS). The Hybrid Multisampler in feed injection mode was used with optimized Feed Speed for the sample injection to trap and enrich the compounds on the column. This avoided peak broadening and breakthrough of the early-eluting polar PFAS compounds. The less polar PFAS compounds can be enriched for more sensitive quantification.

The US EPA has released interim health advisory limits (HALs) of 4 and 20 pg/L in drinking water for the PFAS perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS), respectively. This application note addresses PFAS testing at these challenging new levels in aqueous samples using optimized solid-phase sample extraction and LC/MS/MS analysis with an Agilent 1290 Infinity II liquid chromatography (LC) system coupled to an Agilent 6495C triple quadrupole (TQ) mass spectrometer.

Webinar Date/Time: Wed, Aug 28, 2024 11:00 AM EDT