LCGC North America-11-01-2018

For high performance liquid chromatography (HPLC), ultrahigh-pressure LC (UHPLC), and LC–mass spectrometry (LC–MS) technologies, China has continued to provide solid growth opportunities, as a result of the country’s investments in the pharmaceutical industry, as well as agriculture and food testing.

Click the title above to open the LCGC North America November 2018 issue in an interactive PDF format.

What happens when we inject a sample into the mobile-phase stream? Many LC practitioners are surprised to learn just how serious the effect of the injected sample solvent can be.

Here, we focus on selectivity: its definition, its importance for generating separations and resolution; and its role in column polarity.

We assess the state of the field, first looking back at developments presented at conferences this year, reader questions, and the passing of a pioneer in solid-phase extraction. Then, we look to the future of sample preparation.

The analytical techniques used for characterizing biotherapeutics have evolved. We review the utility of the traditional tools and discuss the new, orthogonal techniques that are increasingly being used.

Scientists executing a method transfer often do not have access to the originating system. Thus, alternative approaches to matching chromatographic results must be considered.

The number of theoretical plates forms the basis of chromatographic theory, and is a key parameter used in all modes of chromatography for measuring column efficiency. Fortunately, it’s easy to measure.