UHPLC

Click here to view the complete E-Separation Solutions newsletter from August 1, 2013.

A summary of the results from a survey of 14 leading HPLC–UHPLC column experts is presented, covering the state of sub-2-?m porous particles, superficially porous (core–shell) particles, silica monoliths, and polymeric monoliths.

The use of UHPLC has triggered a remarkable increase in efficiency and throughput of LC and LC –MS methods. The fastest growing market segment in the pharmaceutical industry is biopharmaceuticals, with an increased interest in applying UHPLC to biopharmaceutical analysis.

Achieving a high quality sample separation is critical to the success of UHPLC analyses. Numerous practical factors must be considered to achieve this.

Market Profile: UHPLC

Sensirion presents a new Nano Flow Sensor for Ultra High Pressure Liquid Chromatography (UHPLC) applications. Key features are a pressure rating of 1000 bar (15000 psi) and a short response time of 30 ms.

Results from research conducted by scientists at Pfizer for a new method for residual active pharmaceutical ingredient (API) analysis have recently been published in a case study by Phenomenex.

HPLC 2008 was held in Baltimore in May. Columnist Ron Majors covers the highlights of this major symposium, held in the U.S. every two years.

We determined enantiomeric ratios of carvone contained in essential oils by achiral ultrahigh-pressure liquid chromatography (UHPLC) with circular dichroism (CD) detection using the g-factor signals.

The use of 30 mm UPLC columns coupled with Oasis SPE in µElution format was investigated to increase the speed of quantitative bioanalytical methods while maintaining sensitivity and resolution of closely related analytes.