February 5th 2025
University of Turin scientists developed a UHPLC–PDA-MS/MS-based method for quantifying plant-specialized metabolites for determining the quality of medicinal and aromatic plants.
Historical Developments in HPLC and UHPLC Column Technology: The Past 25 Years
November 1st 2015During the course of my scientific career beginning in the 1960s, I have grown up with the birth of modern LC column technology, the refinements of the instrumentation, and the development of widespread application of this most powerful separation and analysis technique. In this installment, I would like to share with you some of my observations and experiences with the beginning, the growth period, and the maturation of HPLC columns, where I have focused nearly 33 years of writing for this magazine. I will explore some of the early column breakthroughs beginning with the development of large superficially porous particles (SPP), the porous irregular and spherical microparticulate particles, inorganic and organic polymeric monoliths and the rebirth of the current generation of SPP. In next month’s installment I will look into my crystal ball and see what the future of HPLC and UHPLC holds.
New Chromatography Columns and Accessories for 2015
April 1st 2015Volume 33 Number 4Pages 234-247This is our annual review of new liquid chromatography (LC) columns and accessories introduced at Pittcon and throughout the previous year. This year, Michael Swartz, former author of our "Innovations in HPLC" and "Validation Viewpoint" columns, steps in as a guest columnist to write the review.
Experimental results and method validation data from two analytical case studies of complex small?molecule pharmaceuticals illustrates the utility and advantages of UHPLC in high-resolution separations.
Simultaneous Extraction of Metabolites and Lipids
September 6th 2013A German/Chinese team led by Rainer Lehmann, of the University of Tuebingen (Tuebingen, Germany) and Guowang Xu, of the DICP (Dalian, China), has developed a method of simultaneously extracting metabolites and lipids using methyl tert-butyl ether (MTBE) from single small tissue samples for analysis by ultrahigh-pressure liquid chromatography–mass spectrometry (UHPLC?MS).
The Current Status and Future of LC Column Technology: What the Experts Are Saying
August 1st 2013A summary of the results from a survey of 14 leading HPLC–UHPLC column experts is presented, covering the state of sub-2-?m porous particles, superficially porous (core–shell) particles, silica monoliths, and polymeric monoliths.
How UHPLC and Large Molecules Fit Together
July 5th 2013The use of UHPLC has triggered a remarkable increase in efficiency and throughput of LC and LC –MS methods. The fastest growing market segment in the pharmaceutical industry is biopharmaceuticals, with an increased interest in applying UHPLC to biopharmaceutical analysis.
Utility of UPLC–MS–MS and SPE for High Throughput Quantitative Bioanalysis
September 2nd 2007The use of 30 mm UPLC columns coupled with Oasis SPE in µElution format was investigated to increase the speed of quantitative bioanalytical methods while maintaining sensitivity and resolution of closely related analytes.